一种光亲和性ATP类似物与人红细胞膜阳离子刺激的三磷酸腺苷酶的相互作用。
Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes.
作者信息
Haley B E, Hoffman J F
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3367-71. doi: 10.1073/pnas.71.9.3367.
Abstract
To identify and isolate ATP binding and hydrolyzing sites of human red cell membranes we have synthesized a photo-activated ATP analog, 8-azido adenosine triphosphate (N(3)ATP). In the absence of ultraviolet light it is a substrate for both the Mg-ATPase and the ouabain-sensitive, Na,K-ATPase. Hydrolysis of N(3)ATP is prevented by increasing concentrations of ATP. Photolysis of N(3)ATP with red cell membranes results in covalent incorporation and irreversible inhibition of both ATPase activities. Also, only three protein components of the red cell membranes are labeled. This labeling is completely abolished by appropriate concentrations of ATP.
摘要
为了鉴定和分离人红细胞膜的ATP结合与水解位点，我们合成了一种光活化的ATP类似物，即8-叠氮腺苷三磷酸（N(3)ATP）。在没有紫外线的情况下，它是镁-ATP酶和哇巴因敏感的钠钾-ATP酶的底物。ATP浓度增加可阻止N(3)ATP的水解。N(3)ATP与红细胞膜进行光解反应会导致共价结合并对两种ATP酶活性产生不可逆抑制。此外，红细胞膜中只有三种蛋白质成分被标记。适当浓度的ATP可完全消除这种标记。
相似文献
1
Interactions of a photo-affinity ATP analog with cation-stimulated adenosine triphosphatases of human red cell membranes.一种光亲和性ATP类似物与人红细胞膜阳离子刺激的三磷酸腺苷酶的相互作用。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3367-71. doi: 10.1073/pnas.71.9.3367.
2
Membrane ATP and the functional organization of the red cell Na:K pump.膜ATP与红细胞钠钾泵的功能组织
Ann N Y Acad Sci. 1974;242(0):459-60. doi: 10.1111/j.1749-6632.1974.tb19109.x.
3
Demonstration of a phosphopeptide intermediate in the Mg ++ -dependent, Na + - and K + -stimulated adenosine triphosphatase reaction of the erythrocyte membrane.红细胞膜镁离子依赖、钠离子和钾离子刺激的三磷酸腺苷酶反应中磷酸肽中间体的证实。
Proc Natl Acad Sci U S A. 1972 May;69(5):1216-20. doi: 10.1073/pnas.69.5.1216.
4
The red cell membrane contains three different adenosine triphophatases.
J Biol Chem. 1975 Mar 10;250(5):1952-4.
5
Inhibition of adenosine triphosphatase in sheep red cell membranes by oxidized glutathione.氧化型谷胱甘肽对绵羊红细胞膜中三磷酸腺苷酶的抑制作用。
J Gen Physiol. 1969 Jul;54(1):123-33. doi: 10.1085/jgp.54.1.123.
6
Partial purification of the ouabain-binding component and of Na,K-ATPase from human red cell membranes.从人红细胞膜中对哇巴因结合成分和钠钾ATP酶进行部分纯化。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):936-43. doi: 10.1073/pnas.66.3.936.
7
ATPase and phosphatase activities from human red cell membranes. III. Stimulation of K+-activated phosphatase by phospholipase C.人红细胞膜的ATP酶和磷酸酶活性。III. 磷脂酶C对钾激活磷酸酶的刺激作用
J Membr Biol. 1977 Jun 30;35(2):137-47. doi: 10.1007/BF01869945.
8
The localization of Mg-Na-K-activated adenosine triphosphatase on red cell ghost membranes.镁 - 钠 - 钾激活的三磷酸腺苷酶在红细胞血影膜上的定位。
J Cell Biol. 1967 Nov;35(2):385-404. doi: 10.1083/jcb.35.2.385.
9
The use of aryl azido ATP analogs as photoaffinity labels for myosin ATPase.芳基叠氮基ATP类似物作为肌球蛋白ATP酶光亲和标记物的应用。
J Supramol Struct. 1975;3(5-6):448-68. doi: 10.1002/jss.400030506.
10
The role of bound potassium ions in the hydrolysis of low concentrations of adenosine triphosphate by preparations of membrane fragments from ox brain cerebral cortex.结合钾离子在牛脑大脑皮质膜碎片制剂水解低浓度三磷酸腺苷中的作用。
Biochem J. 1970 Nov;120(1):15-24. doi: 10.1042/bj1200015.
引用本文的文献
1
Sirt1 carboxyl-domain is an ATP-repressible domain that is transferrable to other proteins.Sirt1 羧基结构域是一个可被 ATP 抑制的结构域，并且可以转移到其他蛋白上。
Nat Commun. 2017 May 15;8:15560. doi: 10.1038/ncomms15560.
2
The synthesis and biological properties of 8-azido-N (6)-benzyladenine, a potential photoaffinity reagent for cytokinin.8-叠氮-N（6）-苄基腺嘌呤的合成及生物性质，一种潜在的细胞分裂素光亲和试剂。
Planta. 1977 Jan;137(2):91-6. doi: 10.1007/BF00387544.
3
Photoaffinity labeling of ribulose-bisphosphate carboxylase/oxygenase with 8-azidoadenosine 5'-triphosphate.用 8-叠氮腺苷 5'-三磷酸对核酮糖二磷酸羧化酶/加氧酶进行光亲和标记。
Planta. 1990 Jun;181(3):287-95. doi: 10.1007/BF00195878.
4
Identification of cytoskeletal elements enclosing the ATP pools that fuel human red blood cell membrane cation pumps.鉴定包绕着为人类红细胞膜阳离子泵供能的 ATP 池的细胞骨架元件。
Proc Natl Acad Sci U S A. 2012 Jul 31;109(31):12794-9. doi: 10.1073/pnas.1209014109. Epub 2012 Jun 28.
5
Active cytokinins: photoaffinity labeling agents to detect binding.活性细胞分裂素：用于检测结合的光亲和标记试剂。
Plant Physiol. 1979 Oct;64(4):600-10. doi: 10.1104/pp.64.4.600.
6
Affinity labeling of Avena phytochrome with ATP analogs.用 ATP 类似物对燕麦光敏色素进行亲和标记。
Proc Natl Acad Sci U S A. 1989 May;86(10):3469-73. doi: 10.1073/pnas.86.10.3469.
7
Identification of a common protease-sensitive region in D-alanyl-D-alanine and D-alanyl-D-lactate ligases and photoaffinity labeling with 8-azido ATP.D-丙氨酰-D-丙氨酸和D-丙氨酰-D-乳酸连接酶中常见蛋白酶敏感区域的鉴定以及用8-叠氮基ATP进行光亲和标记。
Protein Sci. 1993 Oct;2(10):1765-9. doi: 10.1002/pro.5560021020.
8
Comparative studies on S-adenosyl-L-methionine binding sites of protein N-methyltransferases, using 8-azido-S-adenosyl-L-methionine as photoaffinity probe.以8-叠氮基-S-腺苷-L-甲硫氨酸作为光亲和探针，对蛋白质N-甲基转移酶的S-腺苷-L-甲硫氨酸结合位点进行的比较研究。
J Protein Chem. 1993 Oct;12(5):603-12. doi: 10.1007/BF01025125.
9
Study of the rat liver S-adenosylmethionine synthetase active site with 8-azido ATP.用8-叠氮基ATP对大鼠肝脏S-腺苷甲硫氨酸合成酶活性位点的研究
Biochem J. 1995 Jun 1;308 ( Pt 2)(Pt 2):565-71. doi: 10.1042/bj3080565.
10
Phosphorylation and dephosphorylation of spectrin from human erythrocyte ghosts under physiological conditions: autocatalysis rather than reaction with separate kinase and phosphatase.生理条件下人红细胞膜血影蛋白的磷酸化与去磷酸化：自身催化而非与单独的激酶和磷酸酶反应。
Proc Natl Acad Sci U S A. 1980 Jun;77(6):3264-8. doi: 10.1073/pnas.77.6.3264.
本文引用的文献
1
PHOSPHATE INCORPORATION AND NA, K-ATPASE ACTIVITY IN HUMAN RED BLOOD CELL GHOSTS.
J Cell Comp Physiol. 1965 Feb;65:31-43. doi: 10.1002/jcp.1030650106.
2
SYNTHESIS OF NUCLEOTIDE ANHYDRIDES BY ANION EXCHANGE.通过阴离子交换合成核苷酸酐。
Biochim Biophys Acta. 1964 Sep 11;91:1-13. doi: 10.1016/0926-6550(64)90164-1.
3
The photolysis of diazoacetylchymotrypsin.重氮乙酰胰凝乳蛋白酶的光解作用。
J Biol Chem. 1962 Sep;237:3006-8.
4
Effects of cysteine and potassium on the ATP-dependent retention of sodium ions by erythrocyte membranes.半胱氨酸和钾对红细胞膜依赖ATP的钠离子潴留的影响。
Biochim Biophys Acta. 1967;135(5):885-93. doi: 10.1016/0005-2736(67)90057-0.
5
Products from the photolysis of diazoacetyl chymotrypsin.重氮乙酰胰凝乳蛋白酶光解产物。
J Biol Chem. 1966 Jan 25;241(2):421-7.
6
A method for marking the hydrophobic binding sites of enzymes. An insertion into the methyl group of an alanine residue of trypsin.一种标记酶疏水结合位点的方法。对胰蛋白酶丙氨酸残基甲基的插入。
J Am Chem Soc. 1969 Jan 1;91(1):217-8. doi: 10.1021/ja01029a055.
7
Photo-affinity labeling of specific acetylcholine-binding sites on membranes.膜上特定乙酰胆碱结合位点的光亲和标记
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1688-94. doi: 10.1073/pnas.67.4.1688.
8
The control of membrane-bound Ca 2+ by ATP.ATP对膜结合Ca²⁺的调控。
Biochim Biophys Acta. 1971 Aug 13;241(2):473-82. doi: 10.1016/0005-2736(71)90046-0.
9
Labeling of the active site of glutamate dehydrogenase with a photogenerated species.用光生成物种标记谷氨酸脱氢酶的活性位点。
Biochem Biophys Res Commun. 1973 Jun 19;52(4):1129-33. doi: 10.1016/0006-291x(73)90617-7.
10
The mechanism of photoaffinity labeling.光亲和标记的机制。
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2567-71. doi: 10.1073/pnas.70.9.2567.
Zotero 插件