一种依赖于线粒体代谢状态的丙酮酸-缬氨酸酶复合物。
A pyruvate-valine enzyme complex that is dependent upon the metabolic state of the mitochondria.
作者信息
Bergquist A, Eakin E A, Murali D K, Wagner R P
出版信息
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4352-5. doi: 10.1073/pnas.71.11.4352.
Abstract
Data are presented suggesting that intact Neurospora crassa mitochondria contain an enzyme complex incorporating five enzymes necessary for the biosynthesis of isoleucine and valine. The functional integrity and stability of the complex has been shown to be dependent on the metabolic state of the mitochondria. The complex has been solubilized by digitonin and has an approximate molecular weight of 400,000.
摘要
所呈现的数据表明，完整的粗糙脉孢菌线粒体含有一种酶复合物，该复合物包含异亮氨酸和缬氨酸生物合成所需的五种酶。已证明该复合物的功能完整性和稳定性取决于线粒体的代谢状态。该复合物已被洋地黄皂苷溶解，其分子量约为400,000。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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