Belloni F L, Phair R D, Sparks H V
Circ Res. 1979 Jun;44(6):759-66. doi: 10.1161/01.res.44.6.759.
A period of prolonged vasodilation follows flow-restricted exercise of skeletal muscle. We tested the hypothesis that adenosine participates in mediating this vascular response. Vascularly isolated, anterior calf muscles of anesthetized dogs were stimulated to contract at a rate of 4 twitches/sec. Blood flow was held constant at 12.5 +/- 1.3 ml/min per 100 g which was about 14% of the expected free flow for this exercise level. Skeletal muscle tissue adenosine was measured with the an enzymatic, spectophotometric assay of trichloroacetic acid extracts of congruent to 50 mg biopsy samples. Tissue adenosine rose from 2.30 +/- 0.90 nmol/g in resting muscle to 22.5 +/- 5.8 nmol/g by the end of the 22-minute exercise. Following exercise, tissue adenosine fell toward its baseline value with a time course very similar to the early portion of the return of skeletal muscle vascular resistance to its control level. Thus, skeletal muscle adenosine content (1) increases to a sufficient magnitude and (2) falls with an appropriate time course to be at least partly responsible for the early portion of prolonged vasodilation seen after flow-restricted exercise of skeletal muscle.
骨骼肌进行血流受限运动后会出现一段长时间的血管舒张期。我们验证了腺苷参与介导这种血管反应的假说。对麻醉犬的小腿前侧肌肉进行血管分离，以每秒4次抽搐的频率刺激其收缩。血流保持恒定，为每100克12.5±1.3毫升/分钟，约为此运动水平预期自由血流的14%。采用酶法分光光度法对约50毫克活检样本的三氯乙酸提取物进行检测，以测定骨骼肌组织中的腺苷。在22分钟运动结束时，组织腺苷从静息肌肉中的2.30±0.90纳摩尔/克升至22.5±5.8纳摩尔/克。运动后，组织腺苷向基线值下降，其时间进程与骨骼肌血管阻力恢复至对照水平早期部分非常相似。因此，骨骼肌腺苷含量（1）升高至足够幅度，（2）并在适当的时间进程下降，至少部分地导致了骨骼肌进行血流受限运动后出现的长时间血管舒张的早期部分。
