Miyamoto H, Kasai M
J Gen Physiol. 1973 Dec;62(6):773-86. doi: 10.1085/jgp.62.6.773.
The effect of direct electrical stimulation on suspensions of sarcoplasmic reticulum membrane fragments (SRF) was carefully re-examined using the method of Lee et al. (1966) J. Gen. Physiol. 49:689. Inhibition of Ca(++) uptake or release by electrical stimulation was observed. When platinum electrodes were used as stimulating electrodes, the effect was dependent on the total current passed through the suspension. On the contrary, when silver-silver chloride electrodes were used, no effect was observed even if voltage and current were the same as in the case of the platinum electrodes. In addition, apparent re-uptake of Ca(++) after cessation of electrical stimulation using platinum electrodes was shown to be due to a binding of Ca(++) to denatured SRF which did not require an energy supply such as ATP, although such re-uptake had been taken as strong evidence of electrical response of SRF in Lee's paper. Finally, it was concluded that the effect of electrical stimulation on SRF was attributable to the irreversible denaturation of SRF due to the oxidation caused by the chlorine generated at the platinum electrode.
采用Lee等人（1966年，《普通生理学杂志》49:689）的方法，对直接电刺激对肌质网膜碎片（SRF）悬浮液的影响进行了仔细的重新研究。观察到电刺激对Ca(++)摄取或释放的抑制作用。当使用铂电极作为刺激电极时，其作用取决于通过悬浮液的总电流。相反，当使用银-氯化银电极时，即使电压和电流与使用铂电极时相同，也未观察到任何作用。此外，使用铂电极停止电刺激后，Ca(++)的明显重新摄取被证明是由于Ca(++)与变性的SRF结合，这种结合不需要ATP等能量供应，尽管在Lee的论文中这种重新摄取被视为SRF电反应的有力证据。最后得出结论，电刺激对SRF的作用归因于铂电极产生的氯所引起的氧化导致的SRF不可逆变性。
