硫酸角质素与蛋白质之间的碱不稳定键。
The alkali-labile linkage between keratan sulphate and protein.
作者信息
Hopwood J J, Robinson H C
出版信息
Biochem J. 1974 Jul;141(1):57-69. doi: 10.1042/bj1410057.
Abstract
Keratan sulphate was isolated from adult intervertebral disc in 90% yield by sequential digestion of the whole tissue with papain, Pronase and Proteus vulgaris chondroitin sulphate lyase. Treatment of this preparation with alkali cleaved a glycosidic bond between N-acetylgalactosamine and threonine and produced, by an alkali-catalysed ;peeling' reaction, an unsaturated derivative of N-acetylgalactosamine which reacted as a chromogen in the Morgan-Elson reaction, but remained covalently bonded to the keratan sulphate chain. This derivative was reduced and labelled by alkaline NaB(3)H(4). The substituent at position 3 of N-acetylgalactosamine in the keratan sulphate-protein linkage was identified as a disaccharide, N-acetylneuraminylgalactose, which was isolated from the reaction mixture after alkali treatment.
摘要
通过用木瓜蛋白酶、链霉蛋白酶和普通变形杆菌硫酸软骨素裂解酶依次消化整个组织，从成人椎间盘中以90%的产率分离出硫酸角质素。用碱处理该制剂会切断N-乙酰半乳糖胺和苏氨酸之间的糖苷键，并通过碱催化的“剥离”反应产生N-乙酰半乳糖胺的不饱和衍生物，该衍生物在摩根-埃尔森反应中作为显色原起反应，但仍与硫酸角质素链共价结合。该衍生物经碱性NaB(3)H(4)还原并标记。硫酸角质素-蛋白质连接中N-乙酰半乳糖胺3位的取代基被鉴定为二糖N-乙酰神经氨酸基半乳糖，它是在碱处理后从反应混合物中分离出来的。
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本文引用的文献
1
Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates.酵母代谢的研究。I. 细胞碳水化合物的分级分离与微量测定。
Biochem J. 1952 Jan;50(3):298-303. doi: 10.1042/bj0500298.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
Aliphatic ammonium salts in the assay of acidic polysaccharides from tissues.组织中酸性多糖测定中的脂肪族铵盐
Methods Biochem Anal. 1960;8:145-97. doi: 10.1002/9780470110249.ch4.
4
VARIATIONS IN KERATOSULFATES.硫酸角质素的变异
J Biol Chem. 1965 Mar;240:1005-10.
5
IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS. XXX. CLEAVAGE OF A, B, AND H BLOOD-GROUP SUBSTANCES BY ALKALI.血型的免疫化学研究。XXX. 用碱裂解A、B和H血型物质
Biochemistry. 1964 Jan;3:113-20. doi: 10.1021/bi00889a018.
6
The determination of amino sugars in the presence of amino acids and glucose.在氨基酸和葡萄糖存在的情况下对氨基糖的测定。
Biochem J. 1960 Dec;77(3):508-10. doi: 10.1042/bj0770508.
7
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.唾液酸的定量测定。II. 比色间苯二酚 - 盐酸法。
Biochim Biophys Acta. 1957 Jun;24(3):604-11. doi: 10.1016/0006-3002(57)90254-8.
8
A modified colorimetric method for the estimation of N-acetylamino sugars.一种用于估算N-乙酰氨基糖的改良比色法。
J Biol Chem. 1955 Dec;217(2):959-66.
9
Qualitative and quantitative determination of mixtures of amino acids using 2,4,6-trinitrobenzenesulfonic acid.使用2,4,6-三硝基苯磺酸对氨基酸混合物进行定性和定量测定。
Anal Biochem. 1967 May;19(2):344-50. doi: 10.1016/0003-2697(67)90170-4.
10
Comparison of human MM, NN, and MN blood group antigens.人类MM、NN和MN血型抗原的比较。
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