软骨蛋白聚糖的碱性裂解和硼氢化钠还原反应
The alkaline cleavage and borohydride reduction of cartilage proteoglycan.
作者信息
Robinson H C, Hopwood J J
出版信息
Biochem J. 1973 Jul;133(3):457-70. doi: 10.1042/bj1330457.
Abstract
A method for the rapid isolation and purification of proteoglycan by using neutral solutions of LiBr for extraction and density-gradient centrifugation is described. The effect of 0.5m-KOH on isolated proteoglycan has been studied by using NaB(3)H(4) to reduce and label the chondroitin sulphate chains released. This study has established: (a) that at least 95% of the chondroitin sulphate chains are attached to the proteoglycan by alkali-labile bonds between xylose and serine; (b) that random degradation of the chondroitin sulphate chains does not occur to any significant extent; (c) that the method is convenient for the determination of polysaccharide number-average molecular weights.
摘要
本文描述了一种通过使用溴化锂中性溶液进行提取和密度梯度离心来快速分离和纯化蛋白聚糖的方法。通过使用硼氢化钠（NaB(3)H(4)）还原并标记释放出的硫酸软骨素链，研究了0.5m - KOH对分离出的蛋白聚糖的影响。该研究确定了：（a）至少95%的硫酸软骨素链通过木糖和丝氨酸之间的碱不稳定键连接到蛋白聚糖上；（b）硫酸软骨素链不会发生显著程度的随机降解；（c）该方法便于测定多糖的数均分子量。
相似文献
1
The alkaline cleavage and borohydride reduction of cartilage proteoglycan.软骨蛋白聚糖的碱性裂解和硼氢化钠还原反应
Biochem J. 1973 Jul;133(3):457-70. doi: 10.1042/bj1330457.
2
Studies on the polydispersity and heterogeneity of cartilage proteoglycans. Identification of 3 proteoglycan structures in bovine nasal cartilage.软骨蛋白聚糖的多分散性和异质性研究。牛鼻软骨中3种蛋白聚糖结构的鉴定。
Biochem J. 1975 Dec;151(3):581-94. doi: 10.1042/bj1510581.
3
Mode of degradation of the chondroitin sulphate proteoglycan in rat costal cartilage.大鼠肋软骨中硫酸软骨素蛋白聚糖的降解模式
Biochem J. 1972 Dec;130(3):729-38. doi: 10.1042/bj1300729.
4
The nature of the protein moieties of cartilage proteoglycans of pig and ox.猪和牛软骨蛋白聚糖中蛋白质部分的性质。
Biochem J. 1975 Sep;149(3):657-68. doi: 10.1042/bj1490657.
5
The composition of cartilage proteoglycans. An investigation using high- and low-inonic-strength extraction procedures.软骨蛋白聚糖的组成。一项使用高离子强度和低离子强度提取程序的研究。
Biochem J. 1973 Mar;131(3):541-53. doi: 10.1042/bj1310541.
6
Multiple aggregation factors in cartilage proteoglycan.软骨蛋白聚糖中的多种聚集因子。
Biochem J. 1973 Jun;133(2):383-6. doi: 10.1042/bj1330383.
7
Age-related changes in the chemical composition of bovine articular cartilage. The structure of high-density proteoglycans.牛关节软骨化学成分的年龄相关性变化。高密度蛋白聚糖的结构。
Biochem J. 1981 Feb 1;193(2):459-68. doi: 10.1042/bj1930459.
8
The effect of beta-D-xylosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage in the absence of protein synthesis inhibitors.在不存在蛋白质合成抑制剂的情况下，β-D-木糖苷对胚胎鸡软骨中硫酸软骨素生物合成的影响。
Biochem J. 1977 Feb 15;162(2):217-33. doi: 10.1042/bj1620217.
9
A novel low-molecular weight chondroitin sulphate proteoglycan isolated from cartilage.从软骨中分离出的一种新型低分子量硫酸软骨素蛋白聚糖。
Biochem J. 1981 Aug 1;197(2):355-66. doi: 10.1042/bj1970355.
10
Isolation and properties of chondroitin sulphates from bovine heart valves.从牛心脏瓣膜中分离硫酸软骨素及其特性
Biochem J. 1970 Jul;118(4):595-601. doi: 10.1042/bj1180595.
引用本文的文献
1
N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions.硫酸乙酰肝素的N-硫酸化对于syndecan-4介导的足细胞与细胞外基质的相互作用至关重要。
Am J Physiol Renal Physiol. 2016 May 15;310(10):F1123-35. doi: 10.1152/ajprenal.00603.2015. Epub 2016 Mar 2.
2
Effect of cycloheximide, beta-D-xylosides and beta-D-galactosides on heparin biosynthesis in mouse mastocytoma.放线菌酮、β-D-木糖苷和β-D-半乳糖苷对小鼠肥大细胞瘤中肝素生物合成的影响。
Biochem J. 1981 Feb 15;194(2):575-86. doi: 10.1042/bj1940575.
3
Control of proteoglycan synthesis. Studies on the activation of synthesis observed during culture of articular cartilages.蛋白聚糖合成的调控。关于关节软骨培养过程中观察到的合成激活的研究。
Biochem J. 1980 Apr 15;188(1):119-30. doi: 10.1042/bj1880119.
4
The structure and composition of cartilage keratan sulphate.软骨硫酸角质素的结构与组成。
Biochem J. 1974 Aug;141(2):517-26. doi: 10.1042/bj1410517.
5
The alkali-labile linkage between keratan sulphate and protein.硫酸角质素与蛋白质之间的碱不稳定键。
Biochem J. 1974 Jul;141(1):57-69. doi: 10.1042/bj1410057.
6
The molecular-weight distribution of glycosaminoglycans.糖胺聚糖的分子量分布
Biochem J. 1973 Dec;135(4):631-7. doi: 10.1042/bj1350631.
7
Constant and variable domains of different disaccharide structure in corneal keratan sulphate chains.角膜硫酸角质素链中不同二糖结构的恒定区和可变区。
Biochem J. 1987 Nov 15;248(1):85-93. doi: 10.1042/bj2480085.
8
The effect of D-xylose, beta-D-xylosides and beta-D-galactosides on chondroitin sulphate biosynthesis in embryonic chicken cartilage.D-木糖、β-D-木糖苷和β-D-半乳糖苷对鸡胚软骨中硫酸软骨素生物合成的影响。
Biochem J. 1975 Apr;148(1):25-34. doi: 10.1042/bj1480025.
9
The synthesis of hyaluronic acid by sheep and rabbit articular cartilage in vitro.绵羊和兔关节软骨体外合成透明质酸。
Biochem J. 1975 Feb;145(2):209-13. doi: 10.1042/bj1450209.
10
The chemical constitution of the proteoglycan of human intervertebral disc.人类椎间盘蛋白聚糖的化学组成。
Biochem J. 1976 Sep 1;157(3):753-63. doi: 10.1042/bj1570753.
本文引用的文献
1
Studies on yeast metabolism. I. Fractionation and microdetermination of cell carbohydrates.酵母代谢的研究。I. 细胞碳水化合物的分级分离与微量测定。
Biochem J. 1952 Jan;50(3):298-303. doi: 10.1042/bj0500298.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE ROLE OF GALACTOSE AND XYLOSE IN THE LINKAGE OF HEPARIN TO PROTEIN.半乳糖和木糖在肝素与蛋白质连接中的作用。
J Biol Chem. 1965 Jul;240:2821-6.
4
VARIATIONS IN KERATOSULFATES.硫酸角质素的变异
J Biol Chem. 1965 Mar;240:1005-10.
5
THE O-SERINE LINKAGE IN PEPTIDES OF CHONDROITIN 4- OR 6-SULFATE.硫酸软骨素4-或6-硫酸酯肽中的O-丝氨酸连接
J Biol Chem. 1965 Jan;240:156-67.
6
EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.稀溶液的平衡超速离心法
Biochemistry. 1964 Mar;3:297-317. doi: 10.1021/bi00891a003.
7
The Lobry de Bruyn-Alberda van Ekenstein transformation.
Adv Carbohydr Chem. 1958;13:63-103. doi: 10.1016/s0096-5332(08)60352-5.
8
The nature of the link between protein and carbohydrate of a chondroitin sulphate complex from hyaline cartilage.来自透明软骨的硫酸软骨素复合物中蛋白质与碳水化合物之间联系的本质。
Biochem J. 1958 Jun;69(2):195-204. doi: 10.1042/bj0690195.
9
Method for the determination of hexosamines in tissues.组织中己糖胺的测定方法。
J Biol Chem. 1953 Oct;204(2):553-63.
10
The simultaneous estimation of 6-deoxy-L-galactose (L-fucose), D-mannose, D-galactose, 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine) and N-acetylneuraminic acid (sialic acid) in glycopeptides and glycoproteins.糖肽和糖蛋白中6-脱氧-L-半乳糖（L-岩藻糖）、D-甘露糖、D-半乳糖、2-乙酰氨基-2-脱氧-D-葡萄糖（N-乙酰-D-葡萄糖胺）和N-乙酰神经氨酸（唾液酸）的同时测定。
Biochim Biophys Acta. 1967 Nov 28;148(2):342-9. doi: 10.1016/0304-4165(67)90129-8.
Zotero 插件