Tom G D, Viswanath-Reddy M, Howe H B
Arch Microbiol. 1978 Jun 26;117(3):259-63. doi: 10.1007/BF00738544.
Specific activities of eight enzymes involved in glycerol metabolism were determined in crude extracts of three strains of Neurospora crassa after growth on six different carbon sources. One of the strains was wild type, which grew poorly on glycerol as sole carbon source; the other two were mutant strains which were efficient glycerol utilizers. A possible basis for this greater efficiency of glycerol utilization was catabolite repression of glyceraldehyde kinase by glycerol in wild type, and two-fold higher glycerate kinase activity in the mutant strains after growth on glycerol, thus apparently allowing two routes for glyceraldehyde to enter the glycolytic pathway in the mutant strains but only one in wild type. The preferential entry of glyceraldehyde to the glycolytic pathway through glycerate was suggested by the lack of glyceraldehyde kinase in all three strains after growth on one or more of the carbon sources and the generally higher levels of aldehyde dehydrogenase and of glycerate kinase than of glyceraldehyde kinase.
在三种粗糙脉孢菌菌株以六种不同碳源生长后的粗提取物中，测定了参与甘油代谢的八种酶的比活性。其中一个菌株是野生型，以甘油作为唯一碳源时生长较差；另外两个是突变菌株，它们是高效的甘油利用者。野生型中甘油对甘油醛激酶的分解代谢阻遏，以及突变菌株在以甘油生长后甘油酸激酶活性高出两倍，这可能是甘油利用效率更高的一个原因，因此突变菌株中甘油醛显然有两条途径进入糖酵解途径，而野生型中只有一条。在以一种或多种碳源生长后，所有三种菌株中均缺乏甘油醛激酶，且醛脱氢酶和甘油酸激酶的水平通常高于甘油醛激酶，这表明甘油醛优先通过甘油酸进入糖酵解途径。
