Holm K, Nilheden E, Kolmark H G
Mol Gen Genet. 1976 Feb 27;144(1):11-15. doi: 10.1007/BF00277297.
Genetic analysis showed that the glycerol non-utilizing isolate gly-u(234) of Neurospora crassa is derived by mutation in a nuclear gene situated in the right arm of linkage group I, about 2.2 cross-over units distal to ad-9 and 11 units proximal to nit-1. Enzymatic testings using a radiochemical method indicate that the mutant is deficient for the enzyme glycerol kinase. The radiochemical testings further indicate that the mutation has inactivated an inducible glycerol kinase, while a low residual activity may be due to a second, basal and non-inducible glycerol kinase, in accordance with a proposal by North (1973, 1974) that Neurospora has two glycerol kinases with these properties.
遗传分析表明，粗糙脉孢菌的甘油非利用型分离株gly-u(234)是由位于连锁群I右臂的一个核基因突变产生的，该基因位于ad-9远端约2.2个交换单位处，nit-1近端11个单位处。使用放射化学方法进行的酶学检测表明，该突变体缺乏甘油激酶。放射化学检测进一步表明，该突变使一种可诱导的甘油激酶失活，而低残留活性可能归因于第二种基础且不可诱导的甘油激酶，这与诺思（1973年、1974年）提出的粗糙脉孢菌有两种具有这些特性的甘油激酶的观点一致。
