Wilson D B
J Bacteriol. 1974 Nov;120(2):866-71. doi: 10.1128/jb.120.2.866-871.1974.
The beta-methyl-galactoside- and galactose-specific transport systems of Escherichia coli were shown by experiments involving inhibitors and the use of an adenosine triphosphatase mutant strain to utilize adenosine 5'-triphosphate or a related compound to drive active transport. These systems were shown to be unable to use the activated-membrane state. The galactose-specific transport system was shown to behave most like a member of the binding-protein class of transport systems by its response to osmotic shock and vesicle formation. These results extended to two sugar transport systems: the correlation between the source of energy and class of transport system found by Berger (1973) for amino acid transport systems. That is, binding-protein systems utilized adenosine 5'-triphosphate whereas membrane-bound systems utilized the activated-membrane state to drive active transport.
通过涉及抑制剂的实验以及使用三磷酸腺苷酶突变菌株,证明大肠杆菌的β-甲基半乳糖苷和半乳糖特异性转运系统利用5'-三磷酸腺苷或相关化合物来驱动主动转运。这些系统被证明无法利用活化膜状态。半乳糖特异性转运系统对渗透休克和囊泡形成的反应表明,它的行为最像转运系统中结合蛋白类的成员。这些结果扩展到两个糖转运系统:Berger(1973年)发现的氨基酸转运系统中能量来源与转运系统类别之间的相关性。也就是说,结合蛋白系统利用5'-三磷酸腺苷,而膜结合系统利用活化膜状态来驱动主动转运。