Kadner R J, Winkler H H
J Bacteriol. 1975 Sep;123(3):985-91. doi: 10.1128/jb.123.3.985-991.1975.
The source of metabolic energy for the accumulation of methionine in cells of Escherichia coli was shown to differ from that for proline uptake. In contrast to proline uptake, methionine accumulation was sensitive to arsenate, and relatively resistant to azide or dinitrophenol. Adenosine triphosphatase mutant strains also differentiated between the two systems, consistent with the conclusion that, although proline uptake is driven directly by the energized membrane state, methionine uptake is not. Methionine transport is similar to that of other osmotic shock-sensitive systems in its direct utilization of adenosine 5'-triphosphate or a related compound as energy source.
已表明,大肠杆菌细胞中蛋氨酸积累的代谢能量来源与脯氨酸摄取的能量来源不同。与脯氨酸摄取不同,蛋氨酸积累对砷酸盐敏感,而对叠氮化物或二硝基苯酚相对抗性较强。三磷酸腺苷酶突变菌株也能区分这两个系统,这与以下结论一致:尽管脯氨酸摄取直接由活跃的膜状态驱动,但蛋氨酸摄取并非如此。蛋氨酸转运在直接利用5'-三磷酸腺苷或相关化合物作为能量来源方面,与其他渗透休克敏感系统相似。