放线菌酮和嘌呤霉素对绿猴肾细胞中猿猴病毒40 T抗原合成的影响。
Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells.
作者信息
Gilden R V, Carp R I
出版信息
J Bacteriol. 1966 Mar;91(3):1295-7. doi: 10.1128/jb.91.3.1295-1297.1966.
Abstract
Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolished when cycloheximide was added up to 10 hr postinfection. In contrast, puromycin, another inhibitor of protein synthesis, did not suppress antigen production. The basis of this differential effect was the inability of puromycin to inhibit protein synthesis in the cells used. This was shown by the failure of the drug to depress the incorporation of labeled amino acid into protein and also failure to inhibit poliovirus synthesis. The puromycin preparation used was very effective in inhibiting poliovirus synthesis in HeLa cells. Thus, appearance of the SV40 T antigen is dependent on protein synthesis in infected cells.
摘要
吉尔登，R. V.（宾夕法尼亚州费城维斯塔研究所）和R. I. 卡普。环己酰亚胺和嘌呤霉素对绿猴肾细胞中猿猴病毒40 T抗原合成的影响。《细菌学杂志》91:1295 - 1297。1966年。——在感染后长达10小时添加环己酰亚胺时，原代非洲绿猴肾细胞中猿猴病毒40（SV40）T抗原的合成被消除。相比之下，另一种蛋白质合成抑制剂嘌呤霉素并未抑制抗原产生。这种差异效应的基础是嘌呤霉素无法抑制所用细胞中的蛋白质合成。这通过该药物未能抑制标记氨基酸掺入蛋白质以及未能抑制脊髓灰质炎病毒合成得以证明。所用的嘌呤霉素制剂在抑制HeLa细胞中的脊髓灰质炎病毒合成方面非常有效。因此，SV40 T抗原的出现取决于感染细胞中的蛋白质合成。
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