氯化镁、半胱氨酸和胰酶对肠道病毒的蚀斑增强作用。
Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin.
作者信息
Wallis C, Morales F, Powell J, Melnick J L
出版信息
J Bacteriol. 1966 May;91(5):1932-5. doi: 10.1128/jb.91.5.1932-1935.1966.
Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Fred Morales, Joycelyn Powell, and Joseph L. Melnick. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. J. Bacteriol. 91:1932-1935. 1966.-Plaque formation of 21 echoviruses (types 1-6, 9, 13, 15-19, 23-26, 29-32) and 8 coxsackieviruses (B1-6, A7, and A9) was enhanced by increased concentrations of MgCl(2), l-cysteine, and pancreatin in agar overlay medium. In most cases, cationic and anionic polymers (diethylaminoethyl dextran, dextran sulfate, or protamine sulfate) were ineffective. All strains of poliovirus and group B coxsackieviruses were enhanced under agar by MgCl(2). Five of the eight coxsackieviruses tested were also enhanced by cysteine or pancreatin. Certain enteroviruses, which have been difficult to assay by plaque method, can now be quantified effectively by incorporation of additives such as MgCl(2), cysteine, or pancreatin into the overlay medium.
沃利斯，克雷格（贝勒医学院，得克萨斯州休斯顿），弗雷德·莫拉莱斯，乔伊斯琳·鲍威尔，以及约瑟夫·L·梅尔尼克。氯化镁、半胱氨酸和胰蛋白酶对肠道病毒蚀斑的增强作用。《细菌学杂志》91:1932 - 1935。1966年。——通过在琼脂覆盖培养基中增加氯化镁、L - 半胱氨酸和胰蛋白酶的浓度，增强了21种埃可病毒（1 - 6型、9型、13型、15 - 19型、23 - 26型、29 - 32型）和8种柯萨奇病毒（B1 - 6型、A7型和A9型）的蚀斑形成。在大多数情况下，阳离子和阴离子聚合物（二乙氨基乙基葡聚糖、硫酸葡聚糖或硫酸鱼精蛋白）无效。所有脊髓灰质炎病毒株和B组柯萨奇病毒在琼脂下都被氯化镁增强。所测试的8种柯萨奇病毒中有5种也被半胱氨酸或胰蛋白酶增强。某些难以通过蚀斑法检测的肠道病毒，现在可以通过在覆盖培养基中加入氯化镁、半胱氨酸或胰蛋白酶等添加剂有效地进行定量。
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