Wallis C, Melnick J L, Rapp F
J Bacteriol. 1966 Jul;92(1):155-60. doi: 10.1128/jb.92.1.155-160.1966.
Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Effects of pancreatin on the growth of reovirus. J. Bacteriol. 92:155-160. 1966.-The influence of pancreatin and other proteolytic enzymes on the growth, plaque formation, and antigenicity of reovirus was studied. Single-cycle yields of virus in the presence of enzyme were not increased, but multiple-cycle yields of virus were greatly enhanced. Immunofluorescence studies demonstrated that the transmission of reovirus from cell to cell is more rapid in the presence of the enzyme. These findings led to the development of a rapid plaque assay system for reovirus, a virus which has previously been difficult to assay by the plaque method. In the recommended procedure, pancreatin is incorporated into the agar overlay. Monkeys immunized with enzyme-treated reovirus yielded higher antibody titers than animals receiving the same amount of untreated virus.
沃利斯，克雷格（得克萨斯州休斯敦贝勒医学院）、约瑟夫·L·梅尔尼克和弗雷德·拉普。胰酶对呼肠孤病毒生长的影响。《细菌学杂志》92:155 - 160。1966年。——研究了胰酶及其他蛋白水解酶对呼肠孤病毒生长、蚀斑形成和抗原性的影响。在酶存在的情况下，病毒的单循环产量未增加，但多循环产量显著提高。免疫荧光研究表明，在酶存在时，呼肠孤病毒在细胞间的传播更快。这些发现促成了一种用于呼肠孤病毒的快速蚀斑测定系统的开发，呼肠孤病毒此前很难用蚀斑法进行测定。在推荐的方法中，将胰酶加入到琼脂覆盖层中。用经酶处理的呼肠孤病毒免疫的猴子产生的抗体效价比接受相同量未处理病毒的动物更高。
