Ackermann W W, Cox D C, Kurtz H, Powers C D, Davies S J
J Bacteriol. 1966 May;91(5):1943-52. doi: 10.1128/jb.91.5.1943-1952.1966.
Ackermann, W. W. (University of Michigan, Ann Arbor), D. C. Cox, H. Kurtz, C. D. Powers, and S. J. Davies. Effect of poliovirus on deoxyribonucleic acid synthesis in HeLa cells. J. Bacteriol. 91:1943-1952. 1966.-Both poliovirus and arginine stimulated deoxyribonucleic acid (DNA) synthesis in cultures of HeLa cells which were preconditioned by incubation in a medium deficient in arginine. However, the number of cells producing DNA was unaffected. DNA synthesis in such preconditioned cells was 10 to 20% of the maximal value obtained with a full complement of amino acids. Inhibition of DNA synthesis was produced in these cultures either by increasing the multiplicity of exposure above 40 plaque-forming units of virus per cell or by increasing the concentration of the deficient amino acid at the time of virus addition. Inhibition of DNA synthesis resulted from a reduction in the fraction of cells producing DNA. The concentration of arginine required for viral inhibition of DNA synthesis is greater than that for viral multiplication.
阿克曼，W.W.（密歇根大学，安阿伯分校），D.C.考克斯，H.库尔茨，C.D.鲍尔斯，以及S.J.戴维斯。脊髓灰质炎病毒对海拉细胞中脱氧核糖核酸合成的影响。《细菌学杂志》91:1943 - 1952。1966年。——脊髓灰质炎病毒和精氨酸都能刺激在缺乏精氨酸的培养基中预培养的海拉细胞培养物中的脱氧核糖核酸（DNA）合成。然而，产生DNA的细胞数量未受影响。在这种预培养的细胞中，DNA合成是在氨基酸完全补充时获得的最大值的10%至20%。在这些培养物中，通过将感染复数增加到每个细胞超过40个噬斑形成单位的病毒，或者在添加病毒时增加缺乏的氨基酸的浓度，都会抑制DNA合成。DNA合成的抑制是由于产生DNA的细胞比例降低所致。病毒抑制DNA合成所需的精氨酸浓度高于病毒增殖所需的浓度。
