DEINHARDT F, BERGS V V, HENLE G, HENLE W
J Exp Med. 1958 Oct 1;108(4):573-89. doi: 10.1084/jem.108.4.573.
Efforts were made to obtain information on some of the quantitative aspects of host cell-virus interactions in MCN cultures persistently infected with Newcastle disease, mumps, and 6-6 viruses, and to elicit the mechanism which permits simultaneous maintenance of virus and cells for indefinite periods of time. It was shown by 4 different technics that only between 10 and less than 1 per cent of the cells yield infectious virus, depending upon the agent employed and, possibly, variations in the conditions of the cultures. No evidence was found to indicate production of non-infectious virus materials. Only the cells carrying infectious virus are capable upon transfer to uninfected cultures to transmit the infection. Cells from persistently infected cultures, which are free of infectious virus at the time of transfer, failed to liberate virus at a later time during incubation periods of up to 4 weeks. The virus-producing cells contain at any given moment not more than 1 infectious unit of virus, suggesting a linear mode of production; i.e., as soon as a virus particle is completed, it is released. Upon inoculation of MCN test tube cultures with chick embryo-adapted NDV persistent infection and interference with vesicular stomatitis virus (VSV) is established with considerable delay. In contrast, following transfer of MCN-adapted NDV, in form of MCN(NDV) cells or first allantoic passage seeds derived therefrom, the number of virus-producing cells increases logarithmically, doubling every 6 to 8 hours until a total of about 10(4) is reached. Thereafter their numbers rise in proportion to the increase in total cell population; i.e., doubling approximately every 48 hours. At the time when 10(4) virus-producing cells are present in the culture interference with VSV is solidly established. In order to obtain this result about 10(6) cells must have adsorbed virus particles, or, in other words, at least 10(6) virus units must have totally been produced instead of the 10(4) measured by infectivity assay. The implications of these and previously reported data have been discussed in detail and a scheme of the course of events in persistently infected cultures has been presented.
人们致力于获取有关在持续感染新城疫、腮腺炎和6-6病毒的MCN培养物中宿主细胞与病毒相互作用的一些定量方面的信息，并找出能使病毒和细胞同时无限期维持的机制。通过4种不同技术表明，根据所用病原体以及培养条件的可能差异，只有10%至不到1%的细胞产生传染性病毒。未发现有非传染性病毒物质产生的证据。只有携带传染性病毒的细胞在转移到未感染的培养物时才能传播感染。来自持续感染培养物的细胞在转移时没有传染性病毒，在长达4周的培养期内后期也未能释放病毒。产生病毒的细胞在任何给定时刻所含病毒不超过1个感染单位，这表明是一种线性产生模式；即一旦病毒粒子形成，就会释放出来。用鸡胚适应的新城疫病毒持续感染接种MCN试管培养物后，与水疱性口炎病毒（VSV）的干扰建立得相当延迟。相比之下，以MCN（NDV）细胞或由此衍生而来 的第一代尿囊液传代种子形式转移适应MCN的新城疫病毒后，产生病毒的细胞数量呈对数增加，每6至8小时翻倍，直到总数达到约10⁴。此后，它们的数量随着总细胞群体的增加而成比例增加；即大约每48小时翻倍。当培养物中有10⁴个产生病毒的细胞时，与VSV的干扰就牢固建立了。为了获得这个结果，大约10⁶个细胞必须吸附了病毒粒子，或者换句话说，必须总共产生至少10⁶个病毒单位，而不是通过感染性测定所测得的10⁴个。这些以及先前报道的数据的含义已进行了详细讨论，并提出了持续感染培养物中事件进程的示意图。
