控制克雷布斯II型小鼠腹水瘤细胞核糖体氨基酸掺入的因素。
Factors controlling amino acid incorporation by ribosomes from krebs II mouse ascites-tumour cells.
作者信息
Kerr I M, Cohen N, Work T S
出版信息
Biochem J. 1966 Mar;98(3):826-35. doi: 10.1042/bj0980826.
Abstract
- A ribosome-cell sap system capable of supporting the incorporation of (14)C-labelled amino acids into protein has been prepared from Krebs II mouse ascites-tumour cells. The requirements of this system for optimum activity and response to added messenger RNA have been investigated. One such system has been obtained for which amino acid incorporation is almost wholly dependent on the addition of suitable messenger RNA. 2. Ribosomes of widely different but predictable activities in the cell-free system have been prepared from Krebs cells pretreated in a variety of ways. The factors in the pretreatment of the cells responsible for these differences have been investigated. 3. The structural and functional properties of these different ribosome preparations and their response to exogenous messenger RNA have been examined and are discussed in the light of modern concepts of the control of protein synthesis.
摘要
- 已从克雷布斯II型小鼠腹水肿瘤细胞中制备出一种核糖体-细胞液系统，该系统能够支持将（14）C标记的氨基酸掺入蛋白质中。已对该系统实现最佳活性的要求以及对添加的信使RNA的反应进行了研究。已获得一种这样的系统，其氨基酸掺入几乎完全依赖于添加合适的信使RNA。2. 已从经过多种方式预处理的克雷布斯细胞中制备出在无细胞系统中具有广泛不同但可预测活性的核糖体。已对细胞预处理中导致这些差异的因素进行了研究。3. 已根据蛋白质合成控制的现代概念，对这些不同核糖体制剂的结构和功能特性及其对外源信使RNA的反应进行了检查并加以讨论。
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