Grossberg S E, Lwoff M, Lwoff A
J Bacteriol. 1966 Nov;92(5):1473-7. doi: 10.1128/jb.92.5.1473-1477.1966.
Grossberg, Sidney E. (Institut Pasteur, Paris, France), Marguerite Lwoff, and André Lwoff. Exaltation of the development of poliovirus by semicarbazide. J. Bacteriol. 92:1473-1477. 1966.-Semicarbazide (NH(2)-NH-CO-NH(2)) remarkably increases the development of poliovirus. In single-cycle experiments, semicarbazide (SCZ) acted to (i) diminish the length of the latent period, (ii) increase the rate of production of virions, and (iii) increase the final yield of virus. These effects were demonstrable in KB cells, a human malignant cell line, and other primate cells. The dose range over which the compound exerts its effect was relatively large, i.e., from 0.3 to 20 mm, whereas higher concentrations inhibited viral growth. SCZ achieved its exalting effect only when it was added during the first 3 hr of viral development; later addition had no effect. Withdrawal of SCZ at intervals during the viral cycle confirmed that its action occurred within 3 hr after infection. The possible mode of action of SCZ is discussed. SCZ appeared to act, at least in part, by increasing the rate of synthesis of the viral ribonucleic acid.
格罗斯伯格，西德尼·E.（法国巴黎巴斯德研究所），玛格丽特·勒沃夫，以及安德烈·勒沃夫。氨基脲对脊髓灰质炎病毒生长的促进作用。《细菌学杂志》92:1473 - 1477。1966年。——氨基脲（NH(2)-NH-CO-NH(2)）能显著促进脊髓灰质炎病毒的生长。在单周期实验中，氨基脲（SCZ）起到了以下作用：（i）缩短潜伏期；（ii）提高病毒粒子的产生速率；（iii）增加病毒的最终产量。这些效应在人恶性细胞系KB细胞以及其他灵长类细胞中均可得到证实。该化合物发挥作用的剂量范围相对较大，即0.3至20毫米，而更高浓度则会抑制病毒生长。SCZ只有在病毒生长的最初3小时内添加才能产生促进作用；之后添加则无效果。在病毒周期中每隔一段时间撤去SCZ证实其作用发生在感染后的3小时内。文中讨论了SCZ可能的作用方式。SCZ似乎至少部分是通过提高病毒核糖核酸的合成速率来发挥作用的。
