Duncan J L, Groman N B
J Exp Med. 1967 Mar 1;125(3):489-500. doi: 10.1084/jem.125.3.489.
It has been demonstrated that a saturating dose of diphtheria toxin produced a 90% inhibition of polio-virus replication in HeLa cells. This inhibition was reflected in infectious viral RNA synthesis and in mature virus production. Toxin had no direct effect on virus particles or I-RNA, and poliovirus adsorption and eclipse appeared to be carried out normally in intoxicated cells. When toxin was given at various time intervals after infection, the amount of inhibition depended on the time of toxin addition. Toxin given before or immediately after infection gave maximum inhibition, while toxin given several hours after infection had little effect. The data suggest that toxin inhibits viral replication through its effect on protein synthesis. It is likely that a critical step in the viral replication cycle, the production of poliovirus-induced RNA polymerase, is inhibited, and possibly the synthesis of capsid protein. Ammonium salts and the aliphatic amines, glycamine and prolamine, prevented the inhibition of viral replication by toxin. The kinetics of the protective action of ammonium chloride and diphtheria antitoxin are remarkably similar.
已经证明，饱和剂量的白喉毒素可使脊髓灰质炎病毒在HeLa细胞中的复制受到90%的抑制。这种抑制反映在感染性病毒RNA的合成以及成熟病毒的产生上。毒素对病毒颗粒或感染性RNA没有直接影响，并且脊髓灰质炎病毒的吸附和隐蔽期在中毒细胞中似乎正常进行。当在感染后的不同时间间隔给予毒素时，抑制程度取决于毒素添加的时间。在感染前或感染后立即给予毒素可产生最大抑制，而在感染数小时后给予毒素则几乎没有效果。数据表明，毒素通过对蛋白质合成的影响来抑制病毒复制。病毒复制周期中的一个关键步骤，即脊髓灰质炎病毒诱导的RNA聚合酶的产生可能受到抑制，衣壳蛋白的合成也可能受到抑制。铵盐以及脂肪族胺类、甘胺和谷胺可防止毒素对病毒复制的抑制。氯化铵和白喉抗毒素的保护作用动力学非常相似。
