Black P H, White B J
J Exp Med. 1967 Apr 1;125(4):629-46. doi: 10.1084/jem.125.4.629.
Primary weanling hamster kidney cultures were transformed with the adeno 2-SV40, adeno 3-SV40, and adeno 12-SV40 hybrid viruses and with adenovirus type 12. The transformed cell lines which were established were characterized with respect to morphology, virus and antigen content, and chromosome aberrations. The adeno 2 and adeno 3-SV40 hybrid transformed cells had the morphology and T antigen content characteristic of SV40 transformations; cells transformed by the former hybrid had cytogenetic changes typical of SV40-transformed cells as well. The adeno 12-SV40 transformed cells were similar morphologically to adeno 12-transformed cells, contained both the SV40 and adeno T antigens and demonstrated the karyotypic instability of SV40-transformed cells, indicating that both viral genomes are operative in these cells. Although the results indicate that the SV40 genome in hybrids derived from the moderately or nononcogenic adenoviruses supplies the determinants for most of the characteristics investigated, and perhaps for oncogenesis, evidence was presented which suggests that a portion of a nononcogenic adenovirus genome may be integrated in adeno 2-SV40 transformed cells and directs the synthesis of adenovirus T antigens.
用腺病毒2-猴空泡病毒40（SV40）、腺病毒3-SV40和腺病毒12-SV40杂交病毒以及腺病毒12对原代断奶仓鼠肾细胞培养物进行转化。对所建立的转化细胞系在形态学、病毒和抗原含量以及染色体畸变方面进行了鉴定。腺病毒2和腺病毒3-SV40杂交转化细胞具有SV40转化所特有的形态学和T抗原含量；由前一种杂交病毒转化的细胞也具有SV40转化细胞典型的细胞遗传学变化。腺病毒12-SV40转化细胞在形态学上与腺病毒12转化细胞相似，同时含有SV40和腺病毒T抗原，并表现出SV40转化细胞的核型不稳定性，这表明两种病毒基因组在这些细胞中均起作用。虽然结果表明，来自中度致癌或非致癌腺病毒的杂交病毒中的SV40基因组为所研究的大多数特征提供了决定因素，也许还为肿瘤发生提供了决定因素，但有证据表明，非致癌腺病毒基因组的一部分可能整合到腺病毒2-SV40转化细胞中，并指导腺病毒T抗原的合成。
