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Biochem J. 1966 Apr;99(1):222-7. doi: 10.1042/bj0990222.
2
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Dokl Akad Nauk SSSR. 1978;242(5):1223-5.
10
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Biochem J. 1968 Jun;108(1):33-40. doi: 10.1042/bj1080033.

引用本文的文献

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Brain Glycogen Structure and Its Associated Proteins: Past, Present and Future.脑糖原结构及其相关蛋白:过去、现在与未来
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3
Metabolism of the reserve polysaccharide of Streptococcus mitis. Some properties of a pullulanase.缓症链球菌储备多糖的代谢。一种支链淀粉酶的某些特性。
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4
A histochemical study of liver enzymes involved in glycogen metabolism in the x-irradiated rat.对受X射线照射大鼠肝脏中参与糖原代谢的酶进行的组织化学研究。
Histochemie. 1971;25(3):247-55. doi: 10.1007/BF00305943.
5
Lack of an alpha-1,4-glucan: alpha-1,4-glucan 6-glycosyl transferase in a case of type IV glycogenosis.IV型糖原贮积症一例中缺乏α-1,4-葡聚糖:α-1,4-葡聚糖6-糖基转移酶
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Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity.纤维溶解丁酸弧菌glgB基因的特性分析,该基因编码一种具有淀粉分解活性的糖原分支酶。
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8
The transfer of mannose to dolichol diphosphate oligosaccharides in pig liver endoplasmic reticulum.甘露糖在猪肝内质网中向二磷酸多萜醇寡糖的转移。
Biochem J. 1975 Nov;152(2):191-9. doi: 10.1042/bj1520191.

本文引用的文献

1
TRANSFER OF GLUCOSYL UNITS TO OLIGOSACCHARIDES AND POLYSACCHARIDES BY THE ACTION OF URIDINE DIPHOSPHOGLUCOSE-ALPHA-GLUCAN TRANSGLUCOSYLASE.通过尿苷二磷酸葡萄糖-α-葡聚糖转葡糖基酶的作用将葡萄糖基单位转移至寡糖和多糖
Biochemistry. 1965 Mar;4:486-95. doi: 10.1021/bi00879a017.
2
ENZYMIC DEGRADATION AND MICRODETERMINATION OF GLYCOGEN.糖原的酶促降解与微量测定
Anal Biochem. 1964 Jan;7:26-36. doi: 10.1016/0003-2697(64)90116-2.
3
alpha-1,4-glucan: alpha-1,4-glucan 6-glycosyltransferase from liver.α-1,4-葡聚糖:来自肝脏的α-1,4-葡聚糖6-糖基转移酶
Biochim Biophys Acta. 1962 Dec 4;65:307-15. doi: 10.1016/0006-3002(62)91049-1.
4
Combined action of oligo-1, 4--1,4-glucantransferase and amylo-1,6-glucosidase in debranching glycogen.寡聚-1,4-1,4-葡聚糖转移酶和淀粉-1,6-葡萄糖苷酶在糖原脱支中的联合作用。
Nature. 1963 Mar 9;197:980-2. doi: 10.1038/197980a0.
5
A new pathway in rabbit muscle for the enzymatic debranching of glycogen.家兔肌肉中糖原酶促脱支的一条新途径。
Nature. 1963 Mar 9;197:979-80. doi: 10.1038/197979a0.
6
Action pattern and specificity of an amylase from Bacillus subtilis.枯草芽孢杆菌淀粉酶的作用模式及特异性
Arch Biochem Biophys. 1963 Mar;100:451-67. doi: 10.1016/0003-9861(63)90112-7.
7
The mechanism of carbohydrase action. 8. Structures of the muscle-phosphorylase limit dextrins of glycogen and amylopectin.碳水化合物酶作用机制。8. 糖原和支链淀粉的肌肉磷酸化酶极限糊精的结构。
Biochem J. 1960 Aug;76(2):264-8. doi: 10.1042/bj0760264.
8
The action of branching enzymes on outer chains of glycogen.分支酶对糖原外链的作用。
J Biol Chem. 1953 Jun;202(2):491-503.
9
Mechanism of amylopectin formation by the action of Q enzyme.Q酶作用下支链淀粉形成的机制。
J Biol Chem. 1952 May;196(2):785-92.
10
Studies on uridine-diphosphate-glucose.尿苷二磷酸葡萄糖的研究。
Biochem J. 1952 Jun;51(3):426-30. doi: 10.1042/bj0510426.

一项关于肝脏分支酶催化反应的研究。

A study of the reaction catalysed by the liver branching enzyme.

作者信息

Verhue W, Hers H G

出版信息

Biochem J. 1966 Apr;99(1):222-7. doi: 10.1042/bj0990222.

DOI:10.1042/bj0990222
PMID:4290551
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1264978/
Abstract

The mechanism of action of liver branching enzyme has been studied by using as substrate two polysaccharides in which the non-reducing ends had been labelled by incubation with phosphorylase and a trace amount of [(14)C]glucose 1-phosphate. After these polysaccharides had been treated with branching enzyme, their structure was analysed by periodate oxidation, by degradation with phosphorylase and amylo-(1-->6)-glucosidase and by degradation with pullulanase. All the results indicate that the branching enzyme catalyses the transfer from (1-->4)- to (1-->6)-linkage of a chain of glucose units, the minimum length of which is six glucose units. A maltodextrin containing 16 glucose units was not acted on by the branching enzyme.

摘要

通过使用两种多糖作为底物来研究肝脏分支酶的作用机制,这两种多糖的非还原端已通过与磷酸化酶和微量[(14)C]葡萄糖1-磷酸一起温育进行了标记。在用分支酶处理这些多糖后,通过高碘酸氧化、用磷酸化酶和淀粉-(1→6)-葡萄糖苷酶降解以及用支链淀粉酶降解来分析它们的结构。所有结果表明,分支酶催化葡萄糖单位链从(1→4)-连接向(1→6)-连接的转移,其最短长度为六个葡萄糖单位。含有16个葡萄糖单位的麦芽糊精不会被分支酶作用。