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非洲猪瘟。3. 利用琼脂双向扩散沉淀试验检测猪组织中的病毒。

African swine fever. 3. The use of the agar double-diffusion precipitation test for the detection of the virus in swine tissue.

作者信息

Boulanger P, Bannister G L, Gray D P, Ruckerbauer G M, Willis N G

出版信息

Can J Comp Med Vet Sci. 1967 Jan;31(1):12-5.

PMID:4291677
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1494621/
Abstract

The agar double-diffusion precipitation test was applied successfully in the demonstration of ASF viral antigen in spleen and liver from swine experimentally infected by the oral route. Positive reactions were obtained with tissues collected as early as 24 hours after the onset of pyrexia and before other clinical manifestation of the disease. Cross-reactions were observed between the various ASF strains used in the study, making the test practical for routine diagnosis in which different strains may be encountered.

摘要

琼脂双向扩散沉淀试验成功应用于检测经口途径实验感染猪的脾脏和肝脏中的非洲猪瘟病毒抗原。早在发热开始后24小时且在该疾病的其他临床表现出现之前采集的组织就获得了阳性反应。在该研究中使用的各种非洲猪瘟病毒株之间观察到了交叉反应,使得该试验适用于可能遇到不同毒株的常规诊断。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b649/1494621/229c54013091/vetsci00002-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b649/1494621/229c54013091/vetsci00002-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b649/1494621/229c54013091/vetsci00002-0020-a.jpg

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引用本文的文献

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African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence.非洲猪瘟。IV. 利用免疫荧光法显示病毒抗原
Can J Comp Med Vet Sci. 1967 Jan;31(1):16-23.
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African swine fever: application of immunoelectroosmophoresis for the detection of antibody.非洲猪瘟:免疫电渗法在抗体检测中的应用
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本文引用的文献

1
Hog Cholera: I. Investigation of the Agar Double-Diffusion Precipitation Test for The Detection of the Virus in Swine Tissue.猪霍乱:一、用于检测猪组织中病毒的琼脂双扩散沉淀试验的研究
Can J Comp Med Vet Sci. 1964 Dec;28(12):297-303.
2
HOG CHOLERA. II. RELIABILITY OF THE AGAR DOUBLE DIFFUSION PRECIPITATION TEST FOR THE DIFFERENTIATION OF H.C. VIRUS FROM OTHER INFECTIOUS AGENTS IN SWINE TISSUE.猪霍乱。二、琼脂双扩散沉淀试验在区分猪霍乱病毒与猪组织中其他传染性病原体方面的可靠性
Can J Comp Med Vet Sci. 1965 Jun;29(6):157-63.
3
PROPAGATION AND MODIFICATION OF AFRICAN SWINE FEVER VIRUS IN CELL CULTURES.
非洲猪瘟病毒在细胞培养中的增殖与变异
Am J Vet Res. 1965 Jan;26:141-6.
4
Serologic and immunologic studies with African swine fever virus.非洲猪瘟病毒的血清学和免疫学研究
Am J Vet Res. 1963 May;24:450-9.
5
Use of agar diffusion precipitation test in the diagnosis of African swine fever.琼脂扩散沉淀试验在非洲猪瘟诊断中的应用。
Am J Vet Res. 1966 Mar;27(117):485-8.
6
African swine fever. II. Detection of the virus in swine tissues by means of the modified direct complement-fixation test.非洲猪瘟。二、通过改良直接补体结合试验检测猪组织中的病毒。
Can J Comp Med Vet Sci. 1967 Jan;31(1):7-11.
7
African swine fever. IV. Demonstration of the viral antigen by means of immunofluorescence.非洲猪瘟。IV. 利用免疫荧光法显示病毒抗原
Can J Comp Med Vet Sci. 1967 Jan;31(1):16-23.