Boulanger P, Bannister G L, Gray D P, Ruckerbauer G M, Willis N G
Can J Comp Med Vet Sci. 1967 Jan;31(1):7-11.
The modified direct complement-fixation test, supplemented with unheated normal calf serum, was used to demonstrate antibodies in sera of swine immunized to African swine fever virus. These antibodies did not react in the ordinary direct non-supplemented complement-fixation test.African swine fever complement-fixing antigen in infected swine tissue is not denatured by extraction with fat solvents. Consequently, good antigens devoid of non-specific reactivity were obtained by extraction with a mixture of acetone and ether. The virus was detected in infected swine tissue harvested one day after beginning of pyrexia. The modified direct complement-fixation test demonstrated cross-reactions between the six strains of virus studied.
改良直接补体结合试验，辅以未加热的正常小牛血清，用于检测接种非洲猪瘟病毒的猪血清中的抗体。这些抗体在普通的直接非补充补体结合试验中不发生反应。感染猪组织中的非洲猪瘟补体结合抗原不会因用脂肪溶剂提取而变性。因此，通过用丙酮和乙醚的混合物提取获得了无非特异性反应的良好抗原。在发热开始后一天采集的感染猪组织中检测到了病毒。改良直接补体结合试验表明所研究的六种病毒株之间存在交叉反应。
