Greig A S, Boulanger P, Bannister G L
Can J Comp Med Vet Sci. 1967 Jan;31(1):24-31.
Six strains of African swine fever (ASF) virus were propagated in culture of primary pig kidney (PK) cells. The course of virus growth was followed by means of the fluorescent antibody staining technique. All 6 strains multiplied in the cultures, and 5 of these eventually showed cytopathic effects leading to cell death. Three of the strains were tested for pathogenicity in pigs at various passage levels. Each showed evidence of modification in virulence after a relatively few passages in PK cells. In one case modified virus produced resistance to challenge with homologous virulent virus. All strains rendered the PK cultures capable of hemadsorption of pig erythrocytes.
六株非洲猪瘟（ASF）病毒在原代猪肾（PK）细胞培养物中增殖。通过荧光抗体染色技术追踪病毒的生长过程。所有6株病毒均在培养物中增殖，其中5株最终表现出细胞病变效应导致细胞死亡。对其中3株病毒在不同传代水平上进行猪致病性测试。每株病毒在PK细胞中经过相对较少的传代后均表现出毒力改变的迹象。在一个案例中，致弱病毒对同源强毒病毒的攻击产生了抗性。所有毒株均使PK培养物能够吸附猪红细胞。
