Fernández-Madrid F
J Cell Biol. 1967 Apr;33(1):27-42. doi: 10.1083/jcb.33.1.27.
Synthesis of collagen on polyribosomes has been demonstrated in vitro in chick embryo corium by radioisotope incorporation, zone centrifugation through sucrose gradients, and analytical ultracentrifugation. Collagen synthesis was associated with polyribosomes ranging in size, as reflected by their sedimentation constants, from about 180S to approximately 1600S. Most of the newly formed collagen, hydroxyproline, was present on the largest polyribosome aggregates ( approximately 350-1600S), but small polyribosomes ( approximately 180-200S) also contained collagen. On the basis of the proline-(14)C/hydroxyproline-(14)C ratios and the disrupting effect of collagenase, the proposal is made that the 350-1600S polyribosomes from this tissue are involved predominantly in collagen synthesis. The large polyribosomes are disrupted extensively by collagenase but only partially by ribonuclease and trypsin. Therefore, it appears that they are stabilized by the interaction of newly forming collagen chains. Evidence is presented consistent with the hypothesis that these large polyribosomes are formed by the aggregation of small polyribosomes (180-200S) through the interaction of collagen polypeptides. It is suggested that these small polyribosomes might be involved in the synthesis of subunits of the collagen alpha chain.
通过放射性同位素掺入、蔗糖密度梯度区带离心和分析超速离心技术，已在体外证实鸡胚真皮的多核糖体上可合成胶原蛋白。胶原蛋白的合成与不同大小的多核糖体有关，其沉降常数反映出大小范围从约180S至约1600S。大多数新形成的胶原蛋白（羟脯氨酸）存在于最大的多核糖体聚集体（约350 - 1600S）上，但小多核糖体（约180 - 200S）也含有胶原蛋白。基于脯氨酸 - （14）C/羟脯氨酸 - （14）C的比例以及胶原酶的破坏作用，有人提出该组织中350 - 1600S的多核糖体主要参与胶原蛋白的合成。大的多核糖体被胶原酶广泛破坏，但仅被核糖核酸酶和胰蛋白酶部分破坏。因此，似乎它们通过新形成的胶原链的相互作用而稳定。有证据支持这样的假说，即这些大的多核糖体是由小多核糖体（180 - 200S）通过胶原多肽的相互作用聚集形成的。有人认为这些小多核糖体可能参与胶原蛋白α链亚基的合成。
