Hertig A T, Adams E C
J Cell Biol. 1967 Aug;34(2):647-75. doi: 10.1083/jcb.34.2.647.
Oocytes in primordial ("resting") follicles in adult human ovaries contain a complex paranuclear structure identified by light microscopists as Balbiani's vitelline body. By electron microscopy this structure is composed of a mass of mitochondria with associated endoplasmic reticulum, multiple compound aggregates which form a ring around the cytocentrum, and a single stack or coil of annulate lamellae either attached to the nuclear membrane or free in the cytoplasm. The compound aggregates contain vacuoles and finely divided electron-opaque material. Evidence is presented for the probable transport of this material between the oocyte and its environment. The cytocentrum contains a central aggregate of amorphous electron-opaque deposits which appear to become periodically aligned on fine fibrils to form the long coarse fibers at the periphery of the cytocentrum. The apparent prevalence of annulate lamellae attached or adjacent to the nuclear membrane of oocytes in ovaries removed during the mid-follicular (estrogenic) phase of the cycle indicates the need for further study of a possible hormonal influence on the resting oocyte. By light microscopy phosphatases were not found within the oocyte, but adenosine-monophosphatase activity is present in the cortical cells surrounding primordial follicles, and also at the periphery of each primitive follicle cell, most prominently at the oocyte side. Glucose-6-phosphate dehydrogenase activity is present within the oocyte cytoplasm.
成年女性卵巢中原始（“静止”）卵泡内的卵母细胞含有一种复杂的核旁结构，光学显微镜学家将其鉴定为巴尔比亚尼卵黄体。通过电子显微镜观察，该结构由一团带有相关内质网的线粒体、围绕细胞中心体形成环状的多个复合聚集体，以及附着于核膜或游离于细胞质中的单排或盘绕的环孔板组成。复合聚集体含有液泡和细分散的电子不透明物质。有证据表明这种物质可能在卵母细胞与其周围环境之间运输。细胞中心体含有一团无定形的电子不透明沉积物，这些沉积物似乎会周期性地排列在细纤维上，形成细胞中心体周边的长粗纤维。在月经周期卵泡中期（雌激素期）切除的卵巢中，卵母细胞核膜附着或相邻的环孔板明显较多，这表明需要进一步研究激素对静止卵母细胞可能产生的影响。通过光学显微镜观察，在卵母细胞内未发现磷酸酶，但在原始卵泡周围的皮质细胞中以及每个原始卵泡细胞的周边存在腺苷一磷酸酶活性，在卵母细胞一侧最为明显。葡萄糖 -6-磷酸脱氢酶活性存在于卵母细胞细胞质中。
