Vance H N
Can J Comp Med Vet Sci. 1967 Oct;31(10):260-4.
Contents of the rumen, abomasum, ileum, and colon of 100 fattened cattle were examined for the presence of Cl. Perfringens. Liquid medium iniculated with each sample of gut content was tested for the presence of toxins of Cl. Perfringens. Identification of Cl. Perfringens was based on atmospheric requirements for growth, colonial morphology, and stormy fermentation in litmus milk. Identification of toxins was based on neutralization tests in guinea pigs and mice.Cl. Perfringens was isolated from 202 of 399 samples. In 105 additional cultures, colonies characteristic of Cl. Perfringens were present but could not be isolated in pure culture.Cl. Perfringens type D toxin was identified in only one culture, which was inoculated with ileum contents. Type A toxin was identified in eight of the 24 samples from the one lot of samples in which no type A antitoxin was used. There were no identifications of toxigenic types B, C, or E. The results indicate that an isolation from necropsy specimens of untyped Cl. Perfringens or type A Cl. Perfringens is in itself of little significance. The infrequency of occurrence of the other toxigenic types in this survey of healthy cattle indicates that recovery of these types from necropsy specimens may be of more significance in determining the cause of death.
对100头育肥牛的瘤胃、皱胃、回肠和结肠内容物进行了产气荚膜梭菌检测。用每份肠道内容物样本接种的液体培养基检测产气荚膜梭菌毒素的存在。产气荚膜梭菌的鉴定基于生长的需氧条件、菌落形态以及石蕊牛奶中的汹涌发酵。毒素的鉴定基于豚鼠和小鼠的中和试验。从399份样本中的202份中分离出产气荚膜梭菌。在另外105份培养物中，存在产气荚膜梭菌特征性菌落，但无法纯培养分离。仅在一份接种回肠内容物的培养物中鉴定出产气荚膜梭菌D型毒素。在未使用A型抗毒素的一批样本中的24份样本中的8份中鉴定出A型毒素。未鉴定出产毒型B、C或E。结果表明，从尸检标本中分离出未分型的产气荚膜梭菌或A型产气荚膜梭菌本身意义不大。在本次健康牛调查中产毒其他类型出现频率较低，这表明从尸检标本中分离出这些类型对于确定死亡原因可能更有意义。
