Deftos L J, Rabson A S, Buckle R M, Aurbach G D, Potts J T
Science. 1968 Jan 26;159(3813):435-6. doi: 10.1126/science.159.3813.435.
Cells froma human parathyroid adenoma were infected with simian virus 40 and maintained through 13 subcultures in monolayer tissue culture. For more than 9 months, these "transformed" cells contimed to produce parathyroid hormone which was identified by radioimmunoassay and density-gradient ultra centrifugation.
用人甲状旁腺腺瘤的细胞感染猿猴病毒40,并在单层组织培养中连续传代13次。在9个多月的时间里,这些“转化”细胞持续产生甲状旁腺激素,该激素通过放射免疫测定和密度梯度超速离心法得以鉴定。