Steinheider G, Greiser-Wilke I, Hauser H, Bumke-Vogt C, Moelling K, Graessmann A
J Virol. 1983 Jan;45(1):375-82. doi: 10.1128/JVI.45.1.375-382.1983.
Thymidine kinase-negative Friend leukemia cells were cotransfected with simian virus 40 (SV40) DNA and thymidine kinase gene DNA of herpes simplex virus type 1. The transfected thymidine kinase-positive cells were selected in HAT medium, and SV40 T-antigen expression was observed over many months in cells cultured under selective conditions, and after adaptation to normal growth medium under nonselective conditions. It was shown by Southern blot hybridization that SV40 DNA was integrated in multiple copies in the chromosomal DNA of several clones. All SV40 DNA-containing Friend leukemia cell clones analyzed were able to undergo induced erythroid differentiation. Induced cultures still expressed SV40 T-antigen to the same extent that untreated control cultures did.
将胸苷激酶阴性的弗氏白血病细胞与猿猴病毒40（SV40）DNA和1型单纯疱疹病毒的胸苷激酶基因DNA共转染。在HAT培养基中选择转染后的胸苷激酶阳性细胞，并在选择性条件下培养的细胞中观察SV40 T抗原表达数月，以及在非选择性条件下适应正常生长培养基后观察其表达情况。Southern印迹杂交显示，SV40 DNA以多拷贝形式整合到几个克隆的染色体DNA中。分析的所有含SV40 DNA的弗氏白血病细胞克隆都能够进行诱导性红细胞分化。诱导培养物中SV40 T抗原的表达程度与未处理的对照培养物相同。
