Olsen T
Scand J Clin Lab Invest. 1979 Feb;39(1):53-9. doi: 10.3109/00365517909104939.
The influence of electrolyte composition and pH of buffer on free T4, free rT3, and free T3 concentrations in serum dialysates has been evaluated, using an equilibrium dialysis method with subsequent direct radioimmunoassay estimations of the dialysate. Cl-, HCO3- and H+ were found to be the important ions which at concentrations similar to those occurring in vivo decreased the protein binding of all three hormones in serum, T3 being much less sensitive to these changes than T4 and rT3. The possible influence of pathological concentrations of electrolytes in serum on estimated free thyroid hormone concentrations is discussed. Because the effect of in vivo changes in serum Cl-, HCO3- and H+ on free thyroid hormone concentrations to a certain extent tend to counteract each other, it is concluded that electrolyte and pH disturbances only to a minor extent can affect measurements on free T4, free rT3 and free T3 concentrations in serum obtained by an equilibrium dialysis method.
采用平衡透析法并随后对透析液进行直接放射免疫分析测定，评估了缓冲液的电解质组成和pH值对血清透析液中游离T4、游离反T3和游离T3浓度的影响。发现Cl-、HCO3-和H+是重要离子，在与体内相似的浓度下，它们会降低血清中所有三种激素的蛋白质结合率，T3对这些变化的敏感性远低于T4和反T3。讨论了血清中电解质的病理浓度对估计的游离甲状腺激素浓度的可能影响。由于体内血清Cl-、HCO3-和H+变化对游离甲状腺激素浓度的影响在一定程度上趋于相互抵消，因此得出结论，电解质和pH值紊乱仅在较小程度上会影响通过平衡透析法获得的血清中游离T4、游离反T3和游离T3浓度的测量结果。
