Total lipid was extracted from Mycoplasma laidlawii strain B with chloroform-methanol mixtures and fractionated into neutral lipid, glycolipid and phospholipid components by chromatography on silicic acid. 2. Saponification of the glycolipid fraction, which represented nearly half of the total lipid, yielded two glycosides for which the structures O-alpha-d-glucopyranosyl-(1-->1)-d-glycerol and O-alpha-d-glucopyranosyl-(1-->2)-O-alpha-d-glucopyranosyl-(1-->1)-d-glycerol were established. 3. The ratio of monoglucosyl diglyceride to diglucosyl diglyceride increased with the age of the culture, though the total glycolipid concentration remained virtually constant. The glycolipid concentration was unaffected by the addition of cholesterol to the culture medium. 4. The phospholipid fraction consisted of two components, phosphatidylglucose and phosphatidylglycerol. Organisms harvested at acidic pH also contained O-amino acyl esters of phosphatidylglycerol. No lipids containing inositol could be detected.
摘要
用氯仿 - 甲醇混合物从莱氏无胆甾原体B株中提取总脂质，并通过在硅酸上进行色谱分离，将其分为中性脂质、糖脂和磷脂成分。2. 糖脂部分（占总脂质近一半）皂化后产生两种糖苷，其结构分别为O-α - d - 吡喃葡萄糖基 - (1→1) - d - 甘油和O-α - d - 吡喃葡萄糖基 - (1→2) - O-α - d - 吡喃葡萄糖基 - (1→1) - d - 甘油。3. 随着培养时间的延长，单葡萄糖二甘油酯与双葡萄糖二甘油酯的比例增加，尽管总糖脂浓度基本保持不变。培养基中添加胆固醇对糖脂浓度没有影响。4. 磷脂部分由磷脂酰葡萄糖和磷脂酰甘油两种成分组成。在酸性pH条件下收获的菌体还含有磷脂酰甘油的O - 氨基酰酯。未检测到含肌醇的脂质。
