The total lipid was extracted from the non-capsulated strain of Pneumococcus I-192R, A.T.C.C. 12213, with chloroform-methanol mixtures. Two glycolipids were isolated by chromatography on silicic acid and DEAE-cellulose (acetate form). 2. The major glycolipid was obtained pure in a yield of 640mg./34g. dry wt. of cells and represents about 34% of the total lipid. It contained galactose, glucose, glycerol and fatty acid ester residues in the proportions 1:1:1:2, and yielded on saponification a crystalline non-reducing glycoside. 3. The structure of the glycoside was shown to be O-alpha-d-galactopyranosyl-(1-->2)-O-alpha-d-glucopyranosyl-(1-->1)-d-glycerol. The fatty acids obtained on saponification were identified by gas-liquid partition chromatography of their methyl esters. 4. The minor glycolipid was obtained as a 1:1 (w/w) mixture with the major component, but after saponification the two glycosides were separated by paper chromatography. Evidence was obtained for the structure of the glycoside derived from the minor glycolipid as 1-O-alpha-d-glucosylglycerol. 5. A general method is described for determining the stereochemistry of the glycerol moiety in 1-linked glycerol glycosides.
摘要
用氯仿 - 甲醇混合物从肺炎球菌I - 192R（美国典型培养物保藏中心12213）的非荚膜菌株中提取总脂质。通过在硅酸和DEAE - 纤维素（醋酸盐形式）上进行色谱分离，分离出两种糖脂。2. 主要糖脂以640mg/34g细胞干重的产率获得纯品，约占总脂质的34%。它含有半乳糖、葡萄糖、甘油和脂肪酸酯残基，比例为1:1:1:2，皂化后产生一种结晶性非还原性糖苷。3. 糖苷的结构显示为O-α - d - 吡喃半乳糖基 - (1→2) - O-α - d - 吡喃葡萄糖基 - (1→1) - d - 甘油。皂化后得到的脂肪酸通过其甲酯的气液分配色谱法进行鉴定。4. 次要糖脂以与主要成分1:1（w/w）的混合物形式获得，但皂化后，两种糖苷通过纸色谱法分离。获得了来自次要糖脂的糖苷结构为1 - O-α - d - 葡糖基甘油的证据。5. 描述了一种用于确定1 - 连接甘油糖苷中甘油部分立体化学的通用方法。
