Akiyama K, Miyamoto T, Horiuchi Y
J Allergy Clin Immunol. 1979 May;63(5):354-60. doi: 10.1016/0091-6749(79)90131-3.
Various methods have been employed for standardization of potency of allergen extracts. We used passive cutaneous anaphylaxis (PCA) inhibition in a mouse-to-rat system as a new means of standardization. Several allergen extracts, including mite, short ragweed, house dust, Aspergillus, Candida, and Japanese cedar, were examined. Mouse IgE antibodies were produced after two or three injections of antigen and alum. The potency measured by PCA inhibition in the mouse-to-rat system was compared with that of protein nitrogen (PN) content, end point of prick test in humans, and 50% radioallergosorbent test (RAST) inhibition. Our studies suggest that the mouse-to-rat system could be used to determine allergenic potency and to standardize allergen extracts.
已经采用了各种方法来标准化变应原提取物的效价。我们在小鼠-大鼠系统中使用被动皮肤过敏反应（PCA）抑制作为一种新的标准化方法。检测了几种变应原提取物，包括螨虫、豚草、屋尘、曲霉、念珠菌和日本柳杉。在注射抗原和明矾两到三次后产生小鼠IgE抗体。将小鼠-大鼠系统中通过PCA抑制测得的效价与蛋白质氮（PN）含量、人体点刺试验终点和50%放射性变应原吸附试验（RAST）抑制进行比较。我们的研究表明，小鼠-大鼠系统可用于确定变应原效价并标准化变应原提取物。
