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评估RAST抑制作为豚草花粉提取物标准化方法的效果。

Evaluation of RAST inhibition as a method for standardization of ragweed pollen extracts.

作者信息

Arbesman C E, Wypych J I, Reisman R E

出版信息

Int Arch Allergy Appl Immunol. 1977;53(4):310-8. doi: 10.1159/000231767.

DOI:10.1159/000231767
PMID:852903
Abstract

The RAST inhibition technique was evaluated as a method for standardization of ragweed pollen extracts. Seven different ragweed extracts were examined. There was no relationship between the RAST inhibition results and the commonly used parameters of extract potency including weight/volume, PNU and total protein measurements. Intradermal skin test end point titration was variable in different patients and could not correlated with RAST inhibition results. However, RAST inhibition potency did correlate with the antigen E content of the extract and this finding suggests that RAST inhibition might provide a method for better standardization of allergen extracts.

摘要

对RAST抑制技术作为豚草花粉提取物标准化方法进行了评估。检测了七种不同的豚草提取物。RAST抑制结果与提取物效力的常用参数(包括重量/体积、PNU和总蛋白测量值)之间没有关系。皮内皮肤试验终点滴定在不同患者中存在差异,且与RAST抑制结果无关。然而,RAST抑制效力确实与提取物的抗原E含量相关,这一发现表明RAST抑制可能为变应原提取物的更好标准化提供一种方法。

相似文献

1
Evaluation of RAST inhibition as a method for standardization of ragweed pollen extracts.评估RAST抑制作为豚草花粉提取物标准化方法的效果。
Int Arch Allergy Appl Immunol. 1977;53(4):310-8. doi: 10.1159/000231767.
2
Brief report: evaluation of polymerized ragweed extracts by intradermal end point titration, RAST inhibition, and parallel line bioassay.简短报告:通过皮内终点滴定、放射变应原吸附试验抑制及平行线生物测定法对聚合豚草提取物进行评估。
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Analysis of the potency of extracts of June grass pollen by their inhibitory capacities in the radioallergosorbent test.
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RAST inhibition: a collaborative study on its suitability and reproducibility for the assay of cocksfoot pollen extracts.放射变应原吸附试验抑制:关于其在测定鸭茅花粉提取物中适用性和可重复性的协作研究。
Clin Allergy. 1986 May;16(3):213-20. doi: 10.1111/j.1365-2222.1986.tb00768.x.
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A new method of allergen standardization. Passive cutaneous anaphylaxis inhibition in a mouse-to-rat system in comparison with other methods.一种变应原标准化的新方法。与其他方法相比,在小鼠 - 大鼠系统中的被动皮肤过敏反应抑制。
J Allergy Clin Immunol. 1979 May;63(5):354-60. doi: 10.1016/0091-6749(79)90131-3.
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Measurement of the potency of pollen extract by radioimmunoprecipitation.通过放射免疫沉淀法测定花粉提取物的效价。
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Evaluation and standardization of polymerized ragweed extracts by chromatography, radioimmunoassay inhibition, and end point cutaneous titration.通过色谱法、放射免疫分析抑制法和终点皮肤滴定法对聚合豚草提取物进行评估和标准化。
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In vivo tests with pollen extracts previously investigated by means of direct RAST titration allergen assay.
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IgE FAST inhibition: a rapid technique for the standardization of pollen allergen extract potency.IgE快速抑制:一种用于花粉变应原提取物效价标准化的快速技术。
Ann Allergy. 1986 Mar;56(3):222-5.

引用本文的文献

1
Allergenic extracts.变应原提取物
Bull N Y Acad Med. 1981 Sep;57(7):549-58.