Balluz S A, Butler M
J Hyg (Lond). 1979 Apr;82(2):285-91. doi: 10.1017/s0022172400025699.
The behaviour of f2 coliphage during activated-sludge treatment was influenced by the temperature, flow-through-time, concentration of mixed liquor suspended solids and the virus load. The most sensitive way to detect behavioural changes was to examine the regression coefficients for the rate of uptake or loss of virus by the mixed liquor solids. This type of analysis revealed, for instance, high values when the solids concentration was high and even greater values occurred when high inocula were used. At high temperature the rate of loss of virus titre after inoculation had stopped was greater than the rate of uptake of virus during inoculation although in all other conditions uptake occurred at a greater rate than the loss of virus. The coefficients were relatively low when the flow rate was increased, when the temperature was low or when the inoculum was small. The distribution of virus between the solids and liquid fractions of the mixed liquor varied somewhat for all conditions but was notably different when (a) the plant was incubated at 5 degrees C when there was much less virus in the solids fraction than usual, and (b) when the inoculum was low and a much higher proportion of virus was found in the solids. The efficiency with which virus was removed across the plant was the least-sensitive determinant of viral behaviour and the value was about the same for most treatment conditions. However, low or high inocula did result in some increased or decreased removal of virus, respectively.
F2 噬菌体在活性污泥处理过程中的行为受到温度、停留时间、混合液悬浮固体浓度和病毒负荷的影响。检测行为变化最敏感的方法是检查混合液固体对病毒摄取或损失速率的回归系数。例如，这种类型的分析表明，当固体浓度高时回归系数值高，当使用高接种量时系数值更高。在高温下，接种停止后病毒滴度的损失速率大于接种期间病毒的摄取速率，尽管在所有其他条件下摄取速率都大于病毒损失速率。当流速增加、温度低或接种量小时，系数相对较低。在所有条件下，混合液中固体和液体部分之间病毒的分布都有所不同，但在以下两种情况下尤为明显：(a) 当装置在 5 摄氏度下培养时，固体部分中的病毒比平常少得多；(b) 当接种量低时，在固体中发现的病毒比例要高得多。整个装置去除病毒的效率是病毒行为最不敏感的决定因素，在大多数处理条件下该值大致相同。然而，低接种量或高接种量确实分别导致病毒去除率有所增加或降低。
