Kitahara M, Eyre H J, Hill H R
J Lab Clin Med. 1979 Mar;93(3):472-9.
Macrophages or monocytes produce CL upon exposure to ingestible particles such as opsonized zymosan or bacteria. In previous studies, we have demonstrated that activated macrophages from mice produce significantly more CL than do normal macrophages. In the present study, we have utilized the CL assay as well as 14C-1-glucose utilization to assess monocyte metabolic activity in a variety of malignant, infectious, and inflammatory diseases. Monocyte peak CL was significantly increased above control values (20.9 +/- 0.5 (S.E.) X 10(3) cpm) in 25 patients with lymphoma (26.7 +/- 1.5 x 10(3)). Markedly increased CL was also seen in inflammatory processes such as bacterial infections, tuberculosis, and sarcoidosis (32.2 +/- 2.7 x 10(3)). In contrast, monocytes from patients with solid tumors, including carcinomas of breast and gastrointestinal and genitourinary tracts, had peak CL values (22.4 +/- 1.6 x 10(3) which were not significantly different from controls. When studied by determining 14C-1-glucose utilization, hexose monophosphate shunt activity paralleled CL values. Monocyte metabolic activation appears therefore to accompany ongoing infectious or granulomatous processes and may also be present in certain malignancies associated with reticuloendothelial stimulation.
巨噬细胞或单核细胞在接触可摄取颗粒（如调理酵母聚糖或细菌）时会产生化学发光（CL）。在先前的研究中，我们已经证明，来自小鼠的活化巨噬细胞比正常巨噬细胞产生的CL显著更多。在本研究中，我们利用CL测定法以及14C-1-葡萄糖利用来评估各种恶性、感染性和炎症性疾病中的单核细胞代谢活性。25例淋巴瘤患者的单核细胞CL峰值显著高于对照值（20.9±0.5（标准误）×10³ 计数/分钟）（26.7±1.5×10³）。在诸如细菌感染、结核病和结节病等炎症过程中也观察到CL显著增加（32.2±2.7×10³）。相比之下，包括乳腺癌、胃肠道癌和泌尿生殖道癌在内的实体瘤患者的单核细胞CL峰值（22.4±1.6×10³）与对照无显著差异。通过测定14C-1-葡萄糖利用进行研究时，磷酸己糖旁路活性与CL值平行。因此，单核细胞代谢活化似乎伴随着正在进行的感染或肉芽肿过程，并且也可能存在于某些与网状内皮系统刺激相关的恶性肿瘤中。
