Wilson G B, Fudenberg H H, Horsmanheimo M
J Lab Clin Med. 1979 May;93(5):800-18.
The effects of DLE containing TFd activity from immune human donors on PBL, obtained from individuals nonresponsive to either PPD or Cocci antigen, were evaluated in vitro by the agarose LMl technique. Several different preparations of DLE were employed to evaluate the specificity and reproducibility of the effects: (1) from donors skin test positive to PPD but negative to Cocci, (2) from donors skin test negative to PPD but positive to Cocci, (3) from donors skin test positive to both antigens, and (4) from donors skin test negative to both antigens. With PBL from other human donors used as target cells in the direct agarose LMi technique, three types of effects were demonstrated for all preparations of DLE: (1) antigen-dependent specific LMl, (2) antigen-independent or nonspecific LMl, and (3) antigen-independent enhancement of migration. The demonstration of each activity was found to depend on the concentration of DLE used and the time allowed for migration. In experiments employing purified PMN and MNL as target cells and a two-step indirect LMl assay, it was shown that the antigen-independent effects resulted from the direct of components in DLE on PMN. The antigen-independent inhibition was shown not to result from toxic effects of DLE. It was produced by DLE but not by dialyzable liver or skin extracts when tested using an amount equivalent to DLE as judged by the absorbance at 260 and 280 nm. The antigen-dependent LMl was found to require secretion of a soluble mediator of molecular weight near 69,000, believed to be LMl. Our results indicate that the agarose LMl technique is a useful in vitro assay for studies of the mechanism of action of components in DLE which can specifically convert nonimmune lymphocytes to a measurable antigen-sensitive state (i.e., transfer factor). The antigen-independent effects of DLE may be responsible in part for previously reported nonspecific beneficial effects of DLE when used in immunotherapy.
采用琼脂糖白细胞移动抑制（LMl）技术，在体外评估了来自对结核菌素纯蛋白衍生物（PPD）或球菌抗原无反应个体的含转移因子（TFd）活性的盘状红斑狼疮提取物（DLE）对人外周血淋巴细胞（PBL）的作用。使用了几种不同的DLE制剂来评估其作用的特异性和可重复性：（1）来自PPD皮肤试验阳性但球菌皮肤试验阴性的供体；（2）来自PPD皮肤试验阴性但球菌皮肤试验阳性的供体；（3）来自两种抗原皮肤试验均阳性的供体；（4）来自两种抗原皮肤试验均阴性的供体。在直接琼脂糖LMl技术中，将来自其他人类供体的PBL用作靶细胞，所有DLE制剂均显示出三种类型的作用：（1）抗原依赖性特异性LMl；（2）抗原非依赖性或非特异性LMl；（3）抗原非依赖性迁移增强。发现每种活性的表现取决于所用DLE的浓度和迁移时间。在使用纯化的多形核白细胞（PMN）和单核白细胞（MNL）作为靶细胞以及两步间接LMl测定的实验中，结果表明抗原非依赖性作用是由DLE中的成分直接作用于PMN所致。抗原非依赖性抑制并非由DLE的毒性作用引起。当使用相当于DLE的量（通过260和280nm处的吸光度判断）进行测试时，它由DLE产生，但不由可透析的肝脏或皮肤提取物产生。发现抗原依赖性LMl需要分泌一种分子量接近69,000的可溶性介质，据信该介质为LMl。我们的结果表明，琼脂糖LMl技术是一种有用的体外测定方法，可以用于研究DLE中能够将非免疫淋巴细胞特异性转化为可测量的抗原敏感状态（即转移因子）的成分的作用机制。DLE的抗原非依赖性作用可能部分解释了先前报道的DLE在免疫治疗中产生的非特异性有益作用。
