Mazaheri R, Hamblin A S, Zuckerman A J
Cell Immunol. 1983 Nov;82(1):147-62. doi: 10.1016/0008-8749(83)90149-1.
Active and adoptive sensitization of rhesus monkeys (Macacca mulatta) as well as the development of a novel sensitive in vitro cell migration inhibition assay for cell-mediated immunity (CMI) in this species are described. First, the correlation of mixed leucocyte-macrophage migration tests (LMMI) with the whole blood lymphocyte transformation (LT) and the delayed hypersensitivity skin test (DH) in immunized animals are shown. Second, these tests are used to demonstrate adoptive transfer of specific/nonspecific cellular immunity (CMI) with dialyzable leucocyte extract (DLE) from immunized donor to unimmunized recipient monkeys. Seventeen animals were immunized with keyhole limpet haemocyanin (KLH) or hepatitis B surface antigen (HBsAg) in Freund's complete adjuvant (FCA) or with FCA alone. Acquisition of antigen-specific cell-mediated immunity was detected by all three tests within 5 weeks of immunization. Positive LMMI responses were associated with positive DH and LT. However, there was no correlation between the magnitude or time of development of the three responses. Therefore, the LMMI test, like the LT test, is an in vitro parameter of DH, but reflects the activity of different subpopulations of lymphocytes and is regulated by different mechanisms. In addition, 12 naive animals received DLE. Within 3 weeks, transfer of sensitivity was detected towards antigens to which the recipients had previously not been reactive but the donors had been. An enhancement of transformation response to phytohaemagglutinin was also seen. Thus, rhesus DLE contains both donor-specific transfer factor-like and nonspecific adjuvant-like activities. In DLE recipients, unlike immunized animals, LMMI responses were dissociated from DH or LT responses in that positive LMMI was mostly seen with negative DH or LT to antigens. Therefore, LMMI emerged as the most sensitive assay for detecting adoptive transfer of CMI by DLE in vivo, supporting the view that different mechanisms regulate LMMI, LT, and DH.
本文描述了恒河猴（猕猴属）的主动致敏和过继致敏，以及开发一种用于该物种细胞介导免疫（CMI）的新型灵敏体外细胞迁移抑制试验。首先，展示了免疫动物中混合白细胞-巨噬细胞迁移试验（LMMI）与全血淋巴细胞转化（LT）以及迟发型超敏皮肤试验（DH）之间的相关性。其次，这些试验用于证明用免疫供体的透析白细胞提取物（DLE）将特异性/非特异性细胞免疫（CMI）过继转移至未免疫的受体猴。17只动物用弗氏完全佐剂（FCA）中的钥孔戚血蓝蛋白（KLH）或乙型肝炎表面抗原（HBsAg）免疫，或仅用FCA免疫。在免疫后5周内，通过所有这三种试验检测到抗原特异性细胞介导免疫的获得。阳性LMMI反应与阳性DH和LT相关。然而，这三种反应的强度或发展时间之间没有相关性。因此，LMMI试验与LT试验一样，是DH的体外参数，但反映了淋巴细胞不同亚群的活性，并受不同机制调节。此外，12只未免疫的动物接受了DLE。在3周内，检测到对受体先前无反应但供体有反应的抗原的敏感性转移。对植物血凝素的转化反应也增强。因此，恒河猴DLE含有类似供体特异性转移因子和类似非特异性佐剂的活性。在接受DLE的动物中，与免疫动物不同，LMMI反应与DH或LT反应分离，因为对抗原的阳性LMMI大多见于阴性DH或LT。因此，LMMI成为检测DLE在体内过继转移CMI的最灵敏试验，支持了不同机制调节LMMI、LT和DH的观点。
