参考肠道病毒的生产。
Production of reference enteroviruses.
作者信息
Kalter S S, Rodriguez A R, Armour V
出版信息
Appl Microbiol. 1968 Oct;16(10):1575-83. doi: 10.1128/am.16.10.1575-1583.1968.
Forty-five human enterovirus reagents of certified purity and quality were prepared for use as seed viruses and as immunizing antigens. One of the reagents was ampouled as "untreated" seed virus, whereas 14 were ampouled as "MgCl(2)-stabilized" reagents. The remaining 30 reagents were ampouled as "untreated" seed viruses and as "MgCl(2)-stabilized" reagents. Thirty of the reagents were propagated on primary African green monkey kidney cells, 3 on primary baboon kidney cells, 3 on primary rhesus monkey kidney cells, and the remaining 9 on human amnion cells. Forty-two of the viral antigens were concentrated for use in the production of high-titered specific antisera in large animals.
制备了45种经认证纯度和质量的人肠道病毒试剂，用作种子病毒和免疫抗原。其中一种试剂以“未处理”种子病毒形式安瓿保存，14种以“MgCl₂稳定化”试剂形式安瓿保存。其余30种试剂分别以“未处理”种子病毒和“MgCl₂稳定化”试剂形式安瓿保存。30种试剂在原代非洲绿猴肾细胞上繁殖，3种在原代狒狒肾细胞上繁殖，3种在原代恒河猴肾细胞上繁殖，其余9种在人羊膜细胞上繁殖。42种病毒抗原进行了浓缩，用于在大型动物中生产高滴度特异性抗血清。
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