Jungwirth C, Launer J
J Virol. 1968 May;2(5):401-8. doi: 10.1128/JVI.2.5.401-408.1968.
Deoxyribonucleic acid (DNA) synthesis was studied in poxvirus-infected cells by measuring (14)C-thymidine incorporation into viral and host cell DNA. A complete separation of the two species of DNA was achieved by combining the previously used "Dounce method" with a separation method based on different reannealing properties of viral and vertebrate DNA. Shortly after infection of HeLa cells with poxviruses, a burst of viral DNA synthesis occurred in the cytoplasm, but a rapid inhibition of host-cell DNA synthesis in the nucleus was observed. This inhibition of cellular DNA synthesis was also found if an accumulation of viral DNA was prevented. At high multiplicites, ultraviolet-irradiated virus inhibited host-cell DNA synthesis to the same extent as fully infectious poxvirus. Under the same conditions, heating at 60 C for 15 min caused a decrease in the ability of cowpox virus to inhibit host-cell DNA synthesis, but did not produce the same effect on vaccinia virus strain WR.
通过测量（14）C-胸腺嘧啶掺入病毒和宿主细胞DNA，研究了痘病毒感染细胞中的脱氧核糖核酸（DNA）合成。将先前使用的“Dounce法”与基于病毒和脊椎动物DNA不同复性特性的分离方法相结合，实现了两种DNA的完全分离。用痘病毒感染HeLa细胞后不久，细胞质中发生了一阵病毒DNA合成，但观察到细胞核中宿主细胞DNA合成迅速受到抑制。如果阻止病毒DNA的积累，也会发现细胞DNA合成受到这种抑制。在高感染复数下，紫外线照射的病毒对宿主细胞DNA合成的抑制程度与完全感染性痘病毒相同。在相同条件下，60℃加热15分钟导致牛痘病毒抑制宿主细胞DNA合成的能力下降，但对痘苗病毒株WR没有产生相同的效果。
