Yano I, Furukawa Y, Kusunose M
J Bacteriol. 1969 Apr;98(1):124-30. doi: 10.1128/jb.98.1.124-130.1969.
The lipids of Nocardia coeliaca were separated into at least 10 components by the use of thin-layer chromatography. Phosphatidylcholine was the most abundant phospholipid in this organism, accounting for 25 to 40% of the total phospholipids. The major fatty acid components of the phosphatidylcholine were 14-methyl-pentadecanoic acid (41%), the other C(15) and C(17) iso- and anteiso-fatty acids (29%), and palmitic acid (13.5%). The next most abundant phospholipid was phosphatidylethanolamine (25 to 30%), followed by phosphatidylinositol (11 to 14%) and cardiolipin (7 to 15%). Phosphatidylethanolamine and phosphatidylinositol were very similar to the phosphatidylcholine in fatty acid composition, whereas cardiolipin was characterized by a higher content of palmitic acid (30%). In all of the phospholipids examined, only trace amounts of monounsaturated fatty acids were present. When washed cells of N. coeliaca were incubated with methionine-methyl-(14)C for 1 to 3 hr, the radioactivity was mainly incorporated into the choline moiety of the phosphatidylcholine. In contrast, acetate-1-(14)C or glycerol-1-(14)C was incorporated much more slowly into the phosphatidylcholine than into the other phospholipids and neutral lipids. No phosphatidylcholine was detected in 10 other species of Nocardia examined.
运用薄层色谱法，将腔孢诺卡氏菌的脂质分离成至少10种成分。磷脂酰胆碱是该生物体中含量最丰富的磷脂，占总磷脂的25%至40%。磷脂酰胆碱的主要脂肪酸成分是14 - 甲基十五烷酸（41%）、其他C(15)和C(17)的异构及反异构脂肪酸（29%）以及棕榈酸（13.5%）。含量次之的磷脂是磷脂酰乙醇胺（25%至30%），其次是磷脂酰肌醇（11%至14%）和心磷脂（7%至15%）。磷脂酰乙醇胺和磷脂酰肌醇在脂肪酸组成上与磷脂酰胆碱非常相似，而心磷脂的特征是棕榈酸含量较高（30%）。在所有检测的磷脂中，仅存在痕量的单不饱和脂肪酸。当将腔孢诺卡氏菌的洗涤细胞与蛋氨酸 - 甲基 - (14)C一起孵育1至3小时时，放射性主要掺入到磷脂酰胆碱的胆碱部分。相比之下，乙酸盐 - 1 - (14)C或甘油 - 1 - (14)C掺入到磷脂酰胆碱中的速度比掺入到其他磷脂和中性脂质中的速度要慢得多。在所检测的其他10种诺卡氏菌中未检测到磷脂酰胆碱。
