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仙台病毒的磷脂和脂肪酸组成特征

Characterization of the phospholipid and fatty acid composition of Sendai virus.

作者信息

Barnes J A, Pehowich D J, Allen T M

出版信息

J Lipid Res. 1987 Feb;28(2):130-7.

PMID:3033116
Abstract

The lipid composition of Sendai virus, propagated in chicken eggs, was analyzed by high performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC). Phosphatidylcholine was found to be the dominant phospholipid (37.3%) with phosphatidylethanolamine (26.8%) and phosphatidylserine (12.0%) also present in significant amounts. Analysis of the fatty acid methyl esters revealed that the dominant fatty acids in total phospholipid were: C16:0 (17.6%), C18:0 (15.4%), C18:1 (n-9) (22.0%), and C24:0 (6.0%). Cardiolipin, phosphatidylserine, and sphingomyelin contained higher levels of saturated fatty acids relative to phosphatidylinositol, phosphatidylethanolamine, and phosphatidylcholine.

摘要

采用高效液相色谱(HPLC)、薄层色谱(TLC)和气液色谱(GLC)分析了在鸡胚中增殖的仙台病毒的脂质组成。发现磷脂酰胆碱是主要的磷脂(37.3%),磷脂酰乙醇胺(26.8%)和磷脂酰丝氨酸(12.0%)的含量也相当可观。脂肪酸甲酯分析表明,总磷脂中的主要脂肪酸为:C16:0(17.6%)、C18:0(15.4%)、C18:1(n-9)(22.0%)和C24:0(6.0%)。相对于磷脂酰肌醇、磷脂酰乙醇胺和磷脂酰胆碱,心磷脂、磷脂酰丝氨酸和鞘磷脂含有更高水平的饱和脂肪酸。

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