Moss C W, Dowell V R, Lewis V J, Schekter M A
J Bacteriol. 1967 Nov;94(5):1300-5. doi: 10.1128/jb.94.5.1300-1305.1967.
A detailed study of the cultural characteristics and cellular fatty acid composition of 27 isolates of Corynebacterium acnes was performed to establish the properties by which this organism may be identified and characterized. The fatty acids were extracted directly from whole cells and examined as methyl esters by gas-liquid chromatography. Each strain possessed a similar fatty acid profile which was characterized by a large percentage of C15 branched-chain acid. Uniformity in certain biochemical reactions and cultural characteristics was also observed. All strains were catalase-positive, nonmotile, and urease-negative, reduced nitrate, liquefied gelatin, failed to hydrolyze esculin and starch, and gave a positive methyl red test. Glucose, fructose, and glycerol were fermented, but not lactose, salicin, sucrose, maltose, xylose, or arabinose. Production of hydrogen sulfide and indole, fermentation of mannitol, and hemolytic activity were variable characteristics. Two species of the genus Propionibacterium were also tested and found to be similar to C. acnes both in cultural characteristics and fatty acid composition. The results strengthen previous suggestions that C. acnes should be classified in the genus Propionibacterium.
对27株痤疮丙酸杆菌的培养特性和细胞脂肪酸组成进行了详细研究，以确定该菌的可识别和鉴定特性。脂肪酸直接从全细胞中提取，并作为甲酯通过气液色谱法进行检测。每株菌都具有相似的脂肪酸谱，其特征是含有大量的C15支链酸。在某些生化反应和培养特性方面也观察到了一致性。所有菌株过氧化氢酶阳性、无动力、脲酶阴性、能还原硝酸盐、能液化明胶、不能水解七叶苷和淀粉，甲基红试验呈阳性。能发酵葡萄糖、果糖和甘油，但不能发酵乳糖、水杨苷、蔗糖、麦芽糖、木糖或阿拉伯糖。硫化氢的产生、吲哚的产生、甘露醇的发酵和溶血活性是可变特征。还对丙酸杆菌属的两个种进行了测试，发现它们在培养特性和脂肪酸组成方面与痤疮丙酸杆菌相似。这些结果强化了之前关于痤疮丙酸杆菌应归入丙酸杆菌属的建议。
