脑心肌炎病毒多聚蛋白在体内的裂解
Cleavage of mengovirus polyproteins in vivo.
作者信息
Lucas-Lenard J
出版信息
J Virol. 1974 Aug;14(2):261-9. doi: 10.1128/JVI.14.2.261-269.1974.
Abstract
The synthesis of mengovirus-specific proteins in vivo was studied by labeling the viral proteins with radioactive amino acids under conditions in which host protein synthesis was almost completely inhibited. Pulse-chase experiments enabled the kinetic analysis of the cleavages of certain viral protein precursors and the formation of others. The pattern of cleavages of mengovirus precursor polypeptides is similar to that of encephalomyocarditis virus. The major difference between the two viruses seems to be in the molar concentration in which the various primary products are produced. The molar ratio of the A protein, which is the precursor of the capsid proteins, to that of the primary products F and C, is approximately 1.5 to 2.0: 1: 1. Possible explanations for the unequal appearance of the structural and nonstructural proteins are discussed.
摘要
通过在宿主蛋白质合成几乎完全受到抑制的条件下用放射性氨基酸标记病毒蛋白，研究了体内脑心肌炎病毒特异性蛋白的合成。脉冲追踪实验能够对某些病毒蛋白前体的切割和其他蛋白的形成进行动力学分析。脑心肌炎病毒前体多肽的切割模式与脑心肌炎病毒相似。这两种病毒之间的主要差异似乎在于各种初级产物产生时的摩尔浓度。作为衣壳蛋白前体的A蛋白与初级产物F和C的摩尔比约为1.5:2.0:1:1。文中讨论了结构蛋白和非结构蛋白出现不均等现象的可能解释。
相似文献
1
Cleavage of mengovirus polyproteins in vivo.脑心肌炎病毒多聚蛋白在体内的裂解
J Virol. 1974 Aug;14(2):261-9. doi: 10.1128/JVI.14.2.261-269.1974.
2
Processing of mengovirus precursor polypeptides in the presence of zinc ions and sulfhydryl compounds.在锌离子和巯基化合物存在的情况下对脑心肌炎病毒前体多肽的加工。
J Virol. 1976 Jun;18(3):918-25. doi: 10.1128/JVI.18.3.918-925.1976.
3
Synthesis of viral-specific polypeptides in Mengo virus-infected L cells: evidence for asymmetric translation of the viral genome.
Virology. 1974 Oct;61(2):315-26. doi: 10.1016/0042-6822(74)90269-4.
4
A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-1A, and poliovirus.脑心肌炎病毒、人鼻病毒1A和脊髓灰质炎病毒的病毒特异性多肽比较
Virology. 1973 Dec;56(2):439-53. doi: 10.1016/0042-6822(73)90048-2.
5
Synthesis and cleavage of virus-specifici proteins in Krebs II carcinoma cells infected with encephalomyocarditis virus.感染脑心肌炎病毒的克雷布斯II型癌细胞中病毒特异性蛋白的合成与裂解
Arch Gesamte Virusforsch. 1972;39(1):98-107. doi: 10.1007/BF01241533.
6
Kinetics of synthesis and cleavage of encephalomyocarditis virus-specific proteins.
Virology. 1972 Nov;50(2):535-49. doi: 10.1016/0042-6822(72)90405-9.
7
Characterization of the large picornaviral polypeptides produced in the presence of zinc ion.锌离子存在下产生的大微小核糖核酸病毒多肽的特性分析。
J Virol. 1974 Aug;14(2):282-91. doi: 10.1128/JVI.14.2.282-291.1974.
8
Capsid polypeptides of mouse Elberfeld virus. I. Amino acid compositions and molar ratios in the virion.小鼠埃尔伯费尔德病毒的衣壳多肽。I. 病毒粒子中的氨基酸组成和摩尔比
J Virol. 1972 Sep;10(3):347-55. doi: 10.1128/JVI.10.3.347-355.1972.
9
Virus-specific proteins synthesized in encephalomyocarditis virus-infected HeLa cells.在脑心肌炎病毒感染的HeLa细胞中合成的病毒特异性蛋白。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3083-7. doi: 10.1073/pnas.68.12.3083.
10
Polypeptides associated with the 250 S mengovirus-induced RNA polymerase structure.与250S脑心肌炎病毒诱导的RNA聚合酶结构相关的多肽
Arch Gesamte Virusforsch. 1974;46(3-4):253-68. doi: 10.1007/BF01240068.
引用本文的文献
1
Ribosomal frameshifting into an overlapping gene in the 2B-encoding region of the cardiovirus genome.核糖体移码进入心脏病毒基因组 2B 编码区的重叠基因。
Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):E1111-9. doi: 10.1073/pnas.1102932108. Epub 2011 Oct 24.
2
Characterization of cricket paralysis virus-induced polypeptides in Drosophila cells.果蝇细胞中蟋蟀麻痹病毒诱导的多肽的特性分析。
J Virol. 1980 Jan;33(1):1-9. doi: 10.1128/JVI.33.1.1-9.1980.
3
Picornaviral structure and assembly.小核糖核酸病毒的结构与组装
Microbiol Rev. 1981 Jun;45(2):287-315. doi: 10.1128/mr.45.2.287-315.1981.
4
Processing of cricket paralysis virus induced polypeptides in Drosophila cells: production of high molecular weight polypeptides by treatment with iodoacetamide.
Arch Virol. 1981;68(1):1-8. doi: 10.1007/BF01315161.
5
The intracellular proteins induced by cricket paralysis virus in Drosophila cells: the effect of protease inhibitors and amino acid analogues.果蝇细胞中蟋蟀麻痹病毒诱导的细胞内蛋白质：蛋白酶抑制剂和氨基酸类似物的作用
Arch Virol. 1981;70(1):1-9. doi: 10.1007/BF01320788.
6
Human rhinovirus protein synthesis and polyprotein cleavage in infected HeLa-RH cells. Brief report.
Arch Virol. 1986;90(3-4):337-42. doi: 10.1007/BF01317381.
7
Regulation of macromolecular synthesis in reovirus-infected L-929 cells I. Effect of L-histidinol.呼肠孤病毒感染的L-929细胞中大分子合成的调控 I. L-组氨醇的作用
J Virol. 1975 Dec;16(6):1503-11. doi: 10.1128/JVI.16.6.1503-1511.1975.
8
Translation of encephalomyocarditis virus RNA in reticulocyte lysates: kinetic analysis of the formation of virion proteins and a protein required for processing.
J Virol. 1979 May;30(2):472-80. doi: 10.1128/JVI.30.2.472-480.1979.
9
Factors affecting composition and thermostability of mengovirus virions.影响脑心肌炎病毒病毒粒子组成和热稳定性的因素。
J Virol. 1977 Mar;21(3):1256-61. doi: 10.1128/JVI.21.3.1256-1261.1977.
10
Evidence of ambiguous processing and selective degradation in the noncapsid proteins of rhinovirus 1A.鼻病毒1A非衣壳蛋白中模糊加工和选择性降解的证据。
J Virol. 1976 Sep;19(3):903-14. doi: 10.1128/JVI.19.3.903-914.1976.
本文引用的文献
1
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
2
Structure of the Mengo virion. I. Polypeptide and ribonucleate components of the virus particle.门戈病毒粒子的结构。I. 病毒颗粒的多肽和核糖核酸成分。
Virology. 1974 Feb;57(2):531-42. doi: 10.1016/0042-6822(74)90192-5.
3
Protein synthesis directed by encephalomyocarditis virus mRNA. 3. Discrete polypeptides translated from a monocistronic messenger in vitro.由脑心肌炎病毒信使核糖核酸指导的蛋白质合成。3. 从单顺反子信使核糖核酸体外翻译出的离散多肽。
Arch Biochem Biophys. 1972 Dec;153(2):706-13. doi: 10.1016/0003-9861(72)90389-x.
4
Initiation of picornavirus protein synthesis in ascites cell extracts.在腹水细胞提取物中启动小核糖核酸病毒蛋白质合成。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3589-93. doi: 10.1073/pnas.69.12.3589.
5
Mechanism of Mengo virus-induced cell injury in L cells: use of inhibitors of protein synthesis to dissociate virus-specific events.门戈病毒诱导L细胞损伤的机制：利用蛋白质合成抑制剂来分离病毒特异性事件。
J Virol. 1972 Nov;10(5):969-78. doi: 10.1128/JVI.10.5.969-978.1972.
6
Kinetics of synthesis and cleavage of encephalomyocarditis virus-specific proteins.
Virology. 1972 Nov;50(2):535-49. doi: 10.1016/0042-6822(72)90405-9.
7
Inhibition of cleavage of large poliovirus-specific precursor proteins in infected HeLa cells by inhibitors of proteolytic enzymes.蛋白水解酶抑制剂对感染的HeLa细胞中大型脊髓灰质炎病毒特异性前体蛋白切割的抑制作用。
J Virol. 1972 Oct;10(4):880-4. doi: 10.1128/JVI.10.4.880-884.1972.
8
Cleavage of viral precursor proteins in vivo and in vitro.病毒前体蛋白在体内和体外的切割
J Virol. 1972 Oct;10(4):751-9. doi: 10.1128/JVI.10.4.751-759.1972.
9
Gene order of encephalomyocarditis virus as determined by studies with pactamycin.通过用 pactamycin 进行的研究确定的脑心肌炎病毒的基因顺序。
J Virol. 1972 May;9(5):823-8. doi: 10.1128/JVI.9.5.823-828.1972.
10
Simple method for the isolation of encephalomyocarditis virus ribonucleic acid.分离脑心肌炎病毒核糖核酸的简易方法。
J Virol. 1972 Mar;9(3):559-61. doi: 10.1128/JVI.9.3.559-561.1972.
Zotero 插件