脑心肌炎病毒多聚蛋白在体内的裂解
Cleavage of mengovirus polyproteins in vivo.
作者信息
Lucas-Lenard J
出版信息
J Virol. 1974 Aug;14(2):261-9. doi: 10.1128/JVI.14.2.261-269.1974.
Abstract
The synthesis of mengovirus-specific proteins in vivo was studied by labeling the viral proteins with radioactive amino acids under conditions in which host protein synthesis was almost completely inhibited. Pulse-chase experiments enabled the kinetic analysis of the cleavages of certain viral protein precursors and the formation of others. The pattern of cleavages of mengovirus precursor polypeptides is similar to that of encephalomyocarditis virus. The major difference between the two viruses seems to be in the molar concentration in which the various primary products are produced. The molar ratio of the A protein, which is the precursor of the capsid proteins, to that of the primary products F and C, is approximately 1.5 to 2.0: 1: 1. Possible explanations for the unequal appearance of the structural and nonstructural proteins are discussed.
摘要
通过在宿主蛋白质合成几乎完全受到抑制的条件下用放射性氨基酸标记病毒蛋白，研究了体内脑心肌炎病毒特异性蛋白的合成。脉冲追踪实验能够对某些病毒蛋白前体的切割和其他蛋白的形成进行动力学分析。脑心肌炎病毒前体多肽的切割模式与脑心肌炎病毒相似。这两种病毒之间的主要差异似乎在于各种初级产物产生时的摩尔浓度。作为衣壳蛋白前体的A蛋白与初级产物F和C的摩尔比约为1.5:2.0:1:1。文中讨论了结构蛋白和非结构蛋白出现不均等现象的可能解释。
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