Frickey P H
Appl Microbiol. 1969 Apr;17(4):589-95. doi: 10.1128/am.17.4.589-595.1969.
Serial passage of the 64-2389 strain of type 3 parainfluenza virus in cercopithecus monkey kidney tissue cultures at low temperatures resulted in the selection of a variant which had a higher efficiency of plaque formation at 25 C than the parent line grown at 37 C. The cold variant, unlike the parent strain, plaqued readily at 25 C, and at 37 C it produced significantly larger plaques. Virus titers of the cold variant in hamster lungs were significantly lower and this was probably caused by the stimulation of interferon by the cold variant during the early phase of the infection. The cold variant, like the virus grown at 37 C, also induced the synthesis of interferon late in the infection. Hamsters responded to the intranasal inoculation of each virus line by the development of hemagglutinating-inhibiting antibodies in the sera.
3型副流感病毒64 - 2389株在低温下于猕猴肾组织培养物中连续传代，筛选出一种变异株，该变异株在25℃时的空斑形成效率高于在37℃生长的亲代株。与亲代毒株不同，冷适应变异株在25℃时易于形成空斑，在37℃时产生的空斑明显更大。冷适应变异株在仓鼠肺中的病毒滴度显著较低，这可能是由于在感染早期冷适应变异株刺激了干扰素的产生。冷适应变异株与在37℃生长的病毒一样，在感染后期也诱导干扰素的合成。仓鼠经鼻接种每种病毒株后，血清中均产生了血凝抑制抗体。
