金属离子对人碱性磷酸酶的正磷酸酶和无机焦磷酸酶活性的影响。
The influence of metal ions on the orthophosphatase and inorganic pyrophosphatase activities of human alkaline phosphatase.
作者信息
Moss D W
出版信息
Biochem J. 1969 May;112(5):699-701. doi: 10.1042/bj1120699.
Abstract
- The differential effects of adding Zn(2+) and Mg(2+) on the orthophosphatase and inorganic pyrophosphatase activities of human intestinal alkaline phosphatase were studied. 2. In the presence of excess of Zn(2+), inorganic pyrophosphatase activity is inhibited. At higher concentrations of pyrophosphate, hydrolysis of this substrate takes place, but is inhibited competitively by the Zn(2+)-pyrophosphate complex. This complex also acts as a competitive inhibitor of orthophosphate hydrolysis. 3. Excess of Mg(2+) also inhibits pyrophosphatase action by removal of substrate; at low concentrations, this ion activates pyrophosphatase, as is the case with orthophosphatase. 4. It is concluded that, when interactions between metal ions and pyrophosphate are taken into account, the effects of these ions are consistent with the view that alkaline phosphatases possess both orthophosphatase and inorganic pyrophosphatase activities.
摘要
- 研究了添加Zn(2+)和Mg(2+)对人肠碱性磷酸酶的正磷酸酶和无机焦磷酸酶活性的不同影响。2. 在过量Zn(2+)存在下，无机焦磷酸酶活性受到抑制。在较高浓度的焦磷酸存在时，该底物会发生水解，但会被Zn(2+)-焦磷酸复合物竞争性抑制。该复合物也作为正磷酸水解的竞争性抑制剂。3. 过量的Mg(2+)也通过去除底物来抑制焦磷酸酶的作用；在低浓度时，这种离子会激活焦磷酸酶，正磷酸酶也是如此。4. 得出的结论是，当考虑金属离子与焦磷酸之间的相互作用时，这些离子的作用与碱性磷酸酶同时具有正磷酸酶和无机焦磷酸酶活性的观点一致。
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