Watson A Y, Brain J D
Lab Invest. 1979 Apr;40(4):450-9.
Spontaneously breathing mice were exposed to an aerosol of iron oxide for 3 hours. Participation of the tracheal and bronchial epithelium in the uptake of iron oxide was noted immediately following the exposure and at 1 day, 4 days, and 7 days postexposure. Observations with the electron microscope revealed that iron oxide was pinocytosed and converted to ferritin and hemosiderin in all epithelial cell types except mucous cells. Iron content increased over time and approximately 50% of the nonmucous cells contained hemosiderin by 4 days postexposure. Ferritin and hemosiderin, but not iron oxide, were noted in connective tissue cells in the submucosa beneath the airway epithelium. Soluble iron and/or ferritin produced in the airway epithelial layer was transported to the submucosa, but normal epithelium prevented the penetration of deposited iron oxide particles to the connective tissue compartment.
将自主呼吸的小鼠暴露于氧化铁气溶胶中3小时。暴露后立即以及暴露后1天、4天和7天，观察到气管和支气管上皮参与了氧化铁的摄取。电子显微镜观察显示，除黏液细胞外，所有上皮细胞类型均通过胞饮作用摄取氧化铁，并将其转化为铁蛋白和含铁血黄素。随着时间的推移，铁含量增加，暴露后4天时，约50%的非黏液细胞含有含铁血黄素。在气道上皮下方黏膜下层的结缔组织细胞中发现了铁蛋白和含铁血黄素，但未发现氧化铁。气道上皮层产生的可溶性铁和/或铁蛋白被转运至黏膜下层，但正常上皮可阻止沉积的氧化铁颗粒穿透至结缔组织间隙。
