卵磷脂多层膜中脂质自旋标记物的取向
Orientation of lipid spin labels in lecithin multilayers.
作者信息
Libertini L J, Waggoner A S, Jost P C, Griffith O H
出版信息
Proc Natl Acad Sci U S A. 1969 Sep;64(1):13-9. doi: 10.1073/pnas.64.1.13.
Abstract
Nitroxide-labeled stearic acid and cholestane have been incorporated into lecithin multilayers. The pronounced anisotropy of the preparations reflects a nonrandom structure of the lecithin film and demonstrates that these lipid probes can report structural information from lipid regions resembling those thought to occur in membranes. These two probes differ with respect to the orientation of the NO bond to the long axis, but both orient with their long axes perpendicular to the phospholipid film. Simulated spectra, calculated on the basis of a Gaussian distribution of orientations, are in agreement with the experimental results.
摘要
氮氧化物标记的硬脂酸和胆甾烷已被掺入卵磷脂多层膜中。制剂明显的各向异性反映了卵磷脂膜的非随机结构，并表明这些脂质探针可以报告来自类似于膜中存在的脂质区域的结构信息。这两种探针在NO键相对于长轴的取向上有所不同，但它们的长轴都垂直于磷脂膜取向。基于取向的高斯分布计算的模拟光谱与实验结果一致。
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