根癌土壤杆菌的己糖醛酸脱氢酶
Hexuronic acid dehydrogenase of Agrobacterium tumefaciens.
作者信息
Chang Y F, Feingold D S
出版信息
J Bacteriol. 1969 Sep;99(3):667-73. doi: 10.1128/jb.99.3.667-673.1969.
Abstract
Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric acid with the concomitant reduction of NAD, but not of nicotinamide adenine dinucleotide phosphate was purified 60-fold by MnCl(2) treatment, (NH(4))(2)SO(4) fractionation, chromatography on diethylaminoethyl Sephadex and negative adsorption with Ca(3)(PO(4))(2) gel. The pH optimum is 8.0. Other uronic acids, aldohexoses, aldopentoses, and polyols, are not substrates. Reduced nicotinamide adenine dinucleotide is an inhibitor strictly competitive with NAD. Kinetic data indicate that the dehydrogenase induced by growth on GlcUA may not be identical with that induced by growth on GalUA.
摘要
根癌土壤杆菌在d-葡萄糖醛酸（GlcUA）或d-半乳糖醛酸（GalUA）上生长会诱导己糖醛酸脱氢酶[D-醛己糖醛酸：烟酰胺腺嘌呤二核苷酸（NAD）氧化还原酶]的形成。该脱氢酶能不可逆地将GlcUA或GalUA转化为相应的己二酸，同时使NAD还原，但不能使烟酰胺腺嘌呤二核苷酸磷酸还原，通过MnCl₂处理、硫酸铵分级分离、二乙氨基乙基葡聚糖凝胶柱色谱和磷酸三钙凝胶负吸附等方法将其纯化了60倍。最适pH为8.0。其他糖醛酸、己醛糖、戊醛糖和多元醇不是底物。还原型烟酰胺腺嘌呤二核苷酸是与NAD严格竞争的抑制剂。动力学数据表明，在GlcUA上生长诱导产生的脱氢酶可能与在GalUA上生长诱导产生的脱氢酶不同。
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