Chang Y F, Feingold D S
J Bacteriol. 1970 Apr;102(1):85-96. doi: 10.1128/jb.102.1.85-96.1970.
Cell-free extract (crude extract) of Agrobacterium tumefaciens grown on d-glucuronate or d-glucarate converts d-glucarate and galactarate to a mixture of 2-keto-3-deoxy- and 4-deoxy-5-keto-d-glucarate. These compounds are then converted by partially purified crude extract to an intermediate tentatively identified as 2,5-diketoadipate. The same enzyme preparation further decarboxylates this intermediate to alpha-ketoglutarate semialdehyde, which is subsequently oxidized in a nicotinamide adenine dinucleotide-dependent reaction to alpha-ketoglutaric acid. Since A. tumefaciens converts d-glucuronic acid to d-glucarate, a pathway from d-glucuronate to alpha-ketoglutarate in A. tumefaciens was determined.
在d-葡萄糖醛酸或d-葡萄糖二酸上生长的根癌土壤杆菌的无细胞提取物（粗提取物）可将d-葡萄糖二酸和半乳糖二酸转化为2-酮-3-脱氧-d-葡萄糖二酸和4-脱氧-5-酮-d-葡萄糖二酸的混合物。然后，这些化合物被部分纯化的粗提取物转化为一种暂定为2,5-二酮己二酸的中间体。相同的酶制剂进一步将该中间体脱羧为α-酮戊二酸半醛，随后α-酮戊二酸半醛在烟酰胺腺嘌呤二核苷酸依赖性反应中被氧化为α-酮戊二酸。由于根癌土壤杆菌可将d-葡萄糖醛酸转化为d-葡萄糖二酸，因此确定了根癌土壤杆菌中从d-葡萄糖醛酸到α-酮戊二酸的途径。
