Strander H
Appl Microbiol. 1969 Nov;18(5):810-5. doi: 10.1128/am.18.5.810-815.1969.
The recovery of interferon from Sendai-infected suspensions of purified human leukocytes is dependent on the serum concentration in the incubation medium. Very little interferon is obtained from serum-free suspensions. The data reported demonstrate that the critical macromolecular, age-independent, and species-unspecific serum principle can be omitted from the suspensions if the medium is supplemented with a combination of crystalline serum albumin and high concentrations of any one of five studied dipolar ionic buffers [N,N-bis(2-hydroxyethyl) glycine (Bicine), N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES), 2-(N-morpholino)ethanesulfonic acid (MES), N-tris(hydroxymethyl)methyl-2-amino-ethanesulfonic acid (TES), and N-tris(hydroxymethyl)methylglycine (Tricine)]. The optimal combination [TES (1.0%, w/v) and bovine serum albumin (0.8%, w/v)] allows the production of potent preparations of serum-free human interferon.
从仙台病毒感染的纯化人白细胞悬液中回收干扰素取决于孵育培养基中的血清浓度。无血清悬液中获得的干扰素很少。所报道的数据表明，如果培养基中添加结晶血清白蛋白和五种研究过的双极性离子缓冲液（N，N-双（2-羟乙基）甘氨酸（Bicine）、N-2-羟乙基哌嗪-N'-2-乙磺酸（HEPES）、2-（N-吗啉代）乙磺酸（MES）、N-三（羟甲基）甲基-2-氨基乙磺酸（TES）和N-三（羟甲基）甲基甘氨酸（Tricine））中的任何一种高浓度组合，则悬液中可省略关键的大分子、与年龄无关且物种非特异性的血清成分。最佳组合（TES（1.0%，w/v）和牛血清白蛋白（0.8%，w/v））可生产出高效的无血清人干扰素制剂。
